Sequences from confirmation amplicon


Confirmation sequences for line 374H09 and hit At5g51270 are shown.
Line ID 374H09
Locus At5g51270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20837526
Primers used for PCR 44rn - 8474, show primer details
   
Sequence Name 65-K115963-0022-374-H09C-44rn
GenBank Accession LN511469 [GenBank]
Sequencing primer gene specific primer, 44rn: 5'-ACTACGTGATGTTTCAGGGCTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K115963-0022-374-H09C-44rn
GAAGACTCTTTTTAGTTCATCTGACTCAGTAGAACTAGTTTCCATAGGAACAGAAACCCC
TCTTACTATTGTCGGGAGATTTTGCATTCTCTTATTGGACAGAGTGTGAGGGTTAGATTT
GAGTGCATTCGACATAACATCTAAAGAATGTACAAAAGAAAAGCGAAGAAAAATCAGACT
CATAAATCACACATCAGTTTCTGGTAAAAAGATTGGTGAAAGCTCCAAACCTGAGTTAGC
TCCTGAACCAGAAGAACAGCTGGAGGAAGTTCTCTCAAAACCAGATTCTATTGAAGTGTC
ATTCCTCTCCGTGTCTGACGTGTCTTTTGCAAGAATATGGACTCCTCCGTTTGAGACAAC
ATAGACTGTGCACAAGTTTGACACAGATGCTGATATCGACGCTGTAATATCATAATTCCT
GGAAGTAGTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC
   
Sequence Name 65-K115962-0022-374-H09C-8409
GenBank Accession LN511468 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K115962-0022-374-H09C-8409
TGATATATCCTGAAACTACTTCCAGGAATTATGATATTACAGCGTCGATATCAGCATCTG
TGTCAAACTTGTGCACAGTCTATGTTGTCTCAAACGGAGGAGTCCATATTCTTGCAAAAG
ACACGTCAGACACGGAGAGGAATGACACTTCAATAGAATCTGGTTTTGAGAGAACTTCCT
CCAGCTGTTCTTCTGGTTCAGGAGCTAACTCAGGTTTGGAGCTTTCACCAATCTTTTTAC
CAGAAACTGATGTGTGATTTATGAGTCTGATTTTTCTTCGCTTTTCTTTTGTACATTCTT
TAGATGTTATGTCGAATGCACTCAAATCTAACCCTCACACTCTGTCCAATAAGAGAATGC
AAAATCTCCCGACAATAGTAAGAGGGGTTTCTGTTCCTATGGAAACTAGTTCTACTGAGT
CAGATGAAACTAAAAAGAGGTCTTCGGATGCTGCAGAGGAAGCAAGCAAGAGAAGTAGCC
CTGAAACTCCACGTAGT

Last Updated on 06.12.2018 16:11