Sequences from confirmation amplicon


Confirmation sequences for line 375B05 and hit At4g32342 are shown.
Line ID 375B05
Locus At4g32342 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15615472
Primers used for PCR 11wu - 8474, show primer details
   
Sequence Name 35-K118761-0022-375-B05C-11wu
GenBank Accession LN502013 [GenBank]
Sequencing primer gene specific primer, 11wu: 5'-AAGTACGTGTGATATGGACCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K118761-0022-375-B05C-11wu
TCTTCAACCCGCCTTTTGATCCCCAATGCAGCACAAGCAACTCTACCGAATTTGTAAACC
ATGGTAACTTTTGAATGTTCTAACTTCTAAGGCCTAGGTTGTTTTGGATGCCAAAAACTT
ATGTTATGAAGCAAGCAGTATTTATTACTCATTCAGTTATTCTATACACTAAAAGATACA
CTACTACTTATTCATTTCCTTCCATAAAAGGACTTATTTTGTGGAACCACACGAGACAAC
AATGGCGTGAATGCTTAACCAGACAACAATGTCTAGTTCCAGAACCTGCAATAAGGTGAC
AACCACAAACAGTTACGAATATTGAACGAACTATTTTTATGTTTATATAGGATTGGTCTA
CTGAATTCGTCAATGTTAAAATGAAACTTTGAATTCATATGATTTCAGCTGGAACTACAT
GGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTATAAT
   
Sequence Name 10-K118760-0022-375-B05C-8409
GenBank Accession LN490881 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118760-0022-375-B05C-8409
CATTTACAATTGATCCATGTAGTTCCAGCTGAAATCATATGAATTCAAAGTTTCATTTTA
ACATTGACGAATTCAGTAGACCAATCCTATATAAACATAAAAATAGTTCGTTCAATATTC
GTAACTGTTTGTGGTTGTCACCTTATTGCAGGTTCTGGAACTAGACATTGTTGTCTGGTT
AAGCATTCACGCCATTGTTGTCTCGTGTGGTTCCACAAAATAAGTCCTTTTATGGAAGGA
AATGAATAAGTAGTAGTGTATCTTTTAGTGTATAGAATAACTGAATGAGTAATAAATACT
GCTTGCTTCATAACATAAGTTTTTGGCATCCAAAACAACCTAGGCCTTAGAAGTTAGAAC
ATTCAAAAGTTACCATGGTTTACAAATTCGGTAGAGTTGCTTGTGCTGCATTGGGGATCA
AAAGGCGGGTTTGAAGATTGGGACTGAATCGTTATATTGTGGTCCATATCACACGTACT

Last Updated on 06.12.2018 16:11