Sequences from confirmation amplicon


Confirmation sequences for line 375B08 and hit At4g32000 are shown.
Line ID 375B08
Locus At4g32000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15475653
Primers used for PCR 84yl - 8474, show primer details
   
Sequence Name 67-K120400-0022-375-B08C-84yl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 84yl: 5'-GATTGTTAAATGTGGGCACTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K120400-0022-375-B08C-84yl
GCATGTGCCATGTTAAAGCCGATCCCCGAGAAGGTCCTTAACCATTAATGATGCAAACAT
TAAATTTTATCAAAACAAAAATTATTTATTTCATGTGGGCACATGATTTGTTTTATCTCT
GTTATACTATAGTATTAATTTAACTCATGATCTATTACACGATGATGAGAAGATGTTTTG
AGCTATTTTTACCGTGTAACTGTGTATCCAATGATCCGCTTTCCATCAGCTCGTAGACGA
TAAAACTCGAACTGAGTTCATTTCCATATCCAAACAATGAGATGATGTTCGGGTGGTGAA
TCTTGCTCAACAAATCAACTTCATTCTGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 43-K120399-0022-375-B08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K120399-0022-375-B08C-8409
AGATTCCCACCCGAACATCATCTCATTGTTTGGATATGGAAATGAACTCAGTTCGAGTTT
TATCGTCTACGAGCTGATGGAAAGCGGATCATTGGATACACAGTTACACGGTAAAAATAG
CTCAAAACATCTTCTCATCATCGTGTAATAGATCATGAGTTAAATTAATACTATAGTATA
ACAGAGATAAAACAAATCATGTGCCCACATGAAATAAATAATTTTTGTTTTGATAAAATT
TAATGTTTGCATCATTAATGGTTAAGGACCTTCTCGGGGATCGGCTTTAACATGGCACAT
GCGGATGAAGATTGCTCTTGATACAGCAAGGTACTACACTCAAGTGCCCACTTTTAACAA
T

Last Updated on 06.12.2018 16:11