Sequences from confirmation amplicon


Confirmation sequences for line 376B09 and hit At3g21230 are shown.
Line ID 376B09
Locus At3g21230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7451387
Primers used for PCR NE35 - 8474, show primer details
   
Sequence Name 63-K102433-0022-376-B09C-8409
GenBank Accession LN510954 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K102433-0022-376-B09C-8409
GCCATTTACAATTGCGTGAGTTTCACGGAACTGACTCAAGCGGACGAAACAGAACTTCTA
AAACCTAAAATATCGCCGGAAGACACGGTGGCGATGCCGTACTCTTCCGGAACAACGGGA
CTTCCAAAGGGAGTGATGATTACTCACAAGGGTTTAGTTACAAGCATCGCTCAGAAAGTC
GACGGAGAAAACCCTAACCTTAACTTCACGGCCAATGACGTCATCCTCTGTTTTCTCCCA
ATGTTTCACATTTACGCTCTCGACGCGTTGATGCTTTCGGCGATGAGAACCGGTGCAGCG
TTACTGATCGTGCCGAGGTTCGAGTTGAATCTGGTGATGGAGCTGATTCAAAGGTACAAA
GTCACGGTTGTGCCGGTGGCTCCTCCGGTGGTTTTAGCATTCATTAAATCCCCTGAGACG
GAGAGATACGACTTGAGCTCGGTGAGAATTATGCTATCAGGTGC
   
Sequence Name 63-K102434-0022-376-B09C-NE35
GenBank Accession LN510955 [GenBank]
Sequencing primer gene specific primer, NE35: 5'-TGCACCTGATAGCATAATTCTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K102434-0022-376-B09C-NE35
GGATTAATGAATGCTAAAACCACCGGAGGAGCCACCGGCACAACCGTGACTTTGTACCTT
TGAATCAGCTCCATCACCAGATTCAACTCGAACCTCGGCACGATCAGTAACGCTGCACCG
GTTCTCATCGCCGAAAGCATCAACGCGTCGAGAGCGTAAATGTGAAACATTGGGAGAAAA
CAGAGGATGACGTCATTGGCCGTGAAGTTAAGGTTAGGGTTTTCTCCGTCGACTTTCTGA
GCGATGCTTGTAACTAAACCCTTGTGAGTAATCATCACTCCCTTTGGAAGTCCCGTTGTT
CCGGAAGAGTACGGCATCGCCACCGTGTCTTCCGGCGATATTTTAGGTTTTAGAAGTTCT
GTTTCGTCCGCTTGAGTCAGTTCCGTGAAACTCACGCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGGG

Last Updated on 06.12.2018 16:11