Sequences from confirmation amplicon


Confirmation sequences for line 377G03 and hit At5g49190 are shown.
Line ID 377G03
Locus At5g49190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19943832
Primers used for PCR RZ18 - 8474, show primer details
   
Sequence Name 37-K020068-022-377-G03C-RZ18
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, RZ18: 5'-ACAGCCAAAACAACATTAACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K020068-022-377-G03C-RZ18
AAAACCCCCCCATTTTTTTTTTCAAAAACCCCCACCAAAAAATTTTAAAAAAGGGGGGGG
AAAAAAAACCCCCCCC
   
Sequence Name 38-K020068-022-377-G03C-8409
GenBank Accession LN502829 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K020068-022-377-G03C-8409
CATGTAGATTTCCCGGACATGAAGCCACATTGACCCATATCATCCAGACCAGGTTGCAGC
TACCTTGGTCAGCTTCTTTGAGACCTGTAACACCAATCCAAATCATTGGGTTAAAATCTC
TGAAGGAGGGCTCAAGCGAATCTATGAAAGGTTGGCCCATTCTCCTTGACAGGCTTAACA
ATACAACTTGTATCGCTTCAACAAGATGATGGCTTAATAAGGATTTTTGCATGTATAGGT
ACACATGGAAGAAGTACTCAGAGAGACTGCTTACCCTGGCTGGAGTCTATGCATTCTGGA
AACATGTGTCTAAGCTCGAAAGGAGAGAAACACGACGTTACCTAGAGATGTTTTACTCAT
TGAAATTTCGTGATTTGGTTAGTGTAACCCACTGTTATTCTTTTGATGTCTACATCTACT
TTACTTACATTATTCTTTTCTTCGGTTTGCAGGCCAATTCAATCCCGCTGGCAACAGATG
AGAACTGATCATGACAGGGTAGATTTTATTTCCTGCACTTTCTTTAGATCTTTTGTTTGT
GTTATCTTGAATAAAAATTGTTGGGTTTTGTTTCCTTCAGTGGTTTGATTTTGGACTTAT
TTGT

Last Updated on 06.12.2018 16:11