Sequences from confirmation amplicon


Confirmation sequences for line 378E02 and hit At1g24800 are shown.
Line ID 378E02
Locus At1g24800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8770428
Primers used for PCR 42yr - 8474, show primer details
   
Sequence Name 34-K122488-0022-378-E02C-42yr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 42yr: 5'-CACTGACTTAGCACGTCACCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K122488-0022-378-E02C-42yr
TTCTGTTTCTTATAGGATTCGTTCTCCAAAGATGGTACCAATGTTGGGATTTTTGTTTAT
TCAATGGAAGATCATTACTCTTGTGAATACAGTAAAATTTGTAACATTAAACTATTTTAG
GTACTAATCATAGGGACATTTTAACCGATCAAACTGTTCCAAAACCAAATTGAATTGAAA
CTTAAGTTGTAGTTCAATAATAGTTTTACTCTGTACCAATTTAACATATTGAAACTGAAA
ATAAAAGAAAATGGAACAGAACTAATAAAAATAATAAAATAATTTAAAACGAAAAAGAAT
TTGACTTGGATACATTTTTCACATTAGGAAAAGGTAAATTCCTCTCCTTTTTTATTTTTA
TTTTTTTACTCGTATTTATAATTGTTGTACATTATAACGTATTAGGATACGTATTAAGAG
AGAAGAACCCTAATTACTTACGAGAGAGGAGAACCCTAATTTTCAATTGTGGTCGAAAAT
TGGAAGAATGAGGAGGAGGAGGTGCGATCTTCAACCGAAGAGAACGAGAATGTGCGATCT
TCAACCGAAGAGAACGAGTATGTGCGATCTTCCACCGAAGCTGGTAGGGGAGAAGATCCT
CACGAGGATTCCGATAACATCTCTGAGAGCGGTGCGATCCACTTGCAAATTATGGAACGC
TTTAACCAAAGATCGGGTTCTGGGTAAAGCAGCGGCGCAGTTTTTGGGGTTTATGACGAT
GGATTCAAAGGTCTGTTCAGTTAGATTCCATCTCCGTCGCAGCAAGGAGGAAGAAGAAGA
CACGATGGATCTATCTATAAAGCAGGTAGATTTGCTTAATCAGTCGAGATATCTAGAGTG
TATCACTG
   
Sequence Name 03-K122487-0022-378-E02C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K122487-0022-378-E02C-oriL
CATTTCATTGAATATATCCTCATGCGCAAAAAGTAGGTATTTCCCTTCACAGAAACGCCG
TAGAGGGGATGATTAGGGTCCATTTCCCCGTTGGGAGTGACCTCGAGAACCCTCCATGAA
TTAGAGCGCAAGTCGTAGATTTCGTACCTGTAAACGGAAACGTTTCTTGTCTGCACCATC
CTCAAGATTTTGTGGTTACGGTTGATGTCGTATCCAAGAGCATAGCTGTCTCCTATGTTG
GATTCTGTTCTGGGTCTGATCCATCTTGTTTGCCCCAAATAAGGGTTCCAAACCACGACC
CTCGAGTTGTCCTTAGCCACGCATAACAACAAGCCGTCGCAGTGATACACTCTAGATATC
TCGACTTGATTAAGCAAATCTACCTGCTTTATAGATAGATCCATCGTGTCTTCTTCTTCC
TCCTTGCTGCGACGGAGATGGAATCTAACTGAACAGACCTTTGAATCCATCGTCATAAAC
CCCAAAAACTGCGCCGCTGCTTTACCCAGAACCCGATCTTTGGTTAAAGCGTTCCATAAT
TTGCAAGTGGATCGCACCGCTCTCAGAGATGTTATCGGAATCCTCGTGAGGATCTTCTCC
CCTACCAGCTTCGGTGGAAGATCGCACATACTCGTTCTCTTCGGTTGAAGATCGCACATT
CTCGTTCTCTTCGGTTGAAGATCGCACCTCCTCCTCCTCATTCTTCCAATTTTCGACCAC
AATTGAAAATTAGGGTTCTCCTCTCTCGTAAGTAATTAGGGTTCTTCTCTCTTAATACGT
ATCCTAATACGTTATAATGTACAACAATTATAAATACGAGTAAAAAAATAAAAATAAAAG
AGGAGAGGAATTTACCTTTTCCTAATGTGAAAAATGTATCCAAGTCAAATTCTTTTTCGT
TTTAAATTATTTTATTATTTTTATTAGTT
Primers used for PCR 57za - 8474, show primer details
   
Sequence Name 23-K123352-0022-378-E02C-57za
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 57za: 5'-AGGTAAGTAGACGAGCAGACAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K123352-0022-378-E02C-57za
CACAGGTTTAAATAAACCATCCTTTCAACTGATAAAAGCGGTGTGGTAACGAGCGCTCTC
GGTGCTTAGGTATCCGTCTAGATCGAAAACCACAGCGACTTTCTCATCCTCGTCAATGAA
GAAGGTGGCACCCATATCATTGTCAAACCGAACACCAGTGAGTGCCAATGGTCTCATATC
CACTGTCAAAAACTTGCTCCAGGACACAGAGTTGGGCTCAATACTAGTAGTAACCCAAAA
CTCAACTGTGGTAAACCGATCATCACTGTGAAGACCCTCATTATGATATAGCACCGCGAG
CTGCTGGTCTCTAACACAAGAGAGGGTCACAGTCGCATCGATAGTAGAGTGAAACGGAAG
AGGCAGACGCAGACCAAATGTCTCTGTTGTAAAATCAAAACAGAGTAAGAAATCTTCAAG
ATCGATTATGTCGCCATCTTCATCAATTTCATCGCCATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAA
   
Sequence Name 07-K123351-0022-378-E02C-8474
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K123351-0022-378-E02C-8474
ACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGGCG
ATGAAATTGATGAAGATGGCGACATAATCGATCTTGAAGATTTCTTACTCTGTTTTGATT
TTACAACAGAGACATTTGGTCTGCGTCTGCCTCTTCCGTTTCACTCTACTATCGATGCGA
CTGTGACCCTCTCTTGTGTTAGAGACCAGCAGCTCGCGGTGCTATATCATAATGAGGGTC
TTCACAGTGATGATCGGTTTACCACAGTTGAGTTTTGGGTTACTACTAGTATTGAGCCCA
ACTCTGTGTCCTGGAGCAAGTTTTTGACAGTGGATATGAGACCATTGGCACTCACTGGTG
TTCGGTTTGACAATGATATGGGTGCCACCTTCTTCATTGACGAGGATGAGAAAGTCGCTG
TGGTTTTCGATCTAGACGGATACCTAAGCACCGAGAGCGCTCGTTACCACACCGCTTTTA
TCAGTGGAAAGGATGGATTCTTTAAACCTGTGACTCTAGGAGTAGCTCCCAATGTCGGGG
AACCTTGTCCCAGAACCGGGCATATTCCGACTACATATCATCCTACACTTGTCTGCTCGT
CTACTTACC

Last Updated on 06.12.2018 16:11