Sequences from confirmation amplicon


Confirmation sequences for line 379D03 and hit At1g72500 are shown.
Line ID 379D03
Locus At1g72500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27296867
Primers used for PCR 39by - 8474, show primer details
   
Sequence Name 80-K108479-0022-379-D03C-39by
GenBank Accession LN513959 [GenBank]
Sequencing primer gene specific primer, 39by: 5'-AAGTTACCTCAACTGATCGGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K108479-0022-379-D03C-39by
GCTCGATCGTTGAAGAACTATCTCAATAGTCTACTCATCTGATGCTCGAATGATTCTGGC
TAGGTGTATTGTTAATACAAAGCCAAGAAATATGTTTTTAGTTTCTTGATTATGAATTAG
TAACACTATCAGCGATAATCAAACAAATAGACCAAAGGAGATTACAATCTACCTGTGTTG
TTTGTGCCGTCATAGTAACCATTCCCTATCCGAGCTAGCATTTGCAAGAAGTAATGGTTG
CAAAACGAACCTGTGGAATTGTTTCTAATCAAAATTTTGAATATTTATATGAAAGAAGCA
AGCAAGTAAAAGCAGAGTATTCTTTTACTTACCAATGCCAAAGGTAGATATCCTAGGAGA
AATCGATTTTCCGTTTCTGCTACAAGATTCTTTCATAGCATGACATATCTCTCTCTCGTT
TTCAACAGATCCATCAGTGACAAGGTATACAAGAGGAACTCCAATATTGCTTCCTTCAAG
TAGTTTCATTGCCTAAACCACACATAGAGAAGAGTCTCAGAGACTAATGTCTCCAGAAAG
ATAAGAGATTCTAGACAACATGTAGAATTACTATTTAATTGAGGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 68-K108478-0022-379-D03C-8409
GenBank Accession LN512031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K108478-0022-379-D03C-8409
TCATTATAGTAATTCTCATGTTGTCTAGAATCTCTTATCTTTCTGGAGACATTAGTCTCT
GAGACTCTTCTCTATGTGTGGTTTAGGCAATGAAACTACTTGAAGGAAGCAATATTGGAG
TTCCTCTTGTATACCTTGTCACTGATGGATCTGTTGAAAACGAGAGAGAGATATGTCATG
CTATGAAAGAATCTTGTAGCAGAAACGGAAAATCGATTTCTCCTAGGATATCTACCTTTG
GCATTGGTAAGTAAAAGAATACTCTGCTTTTACTTGCTTGCTTCTTTCATATAAATATTC
AAAATTTTGATTAGAAACAATTCCACAGGTTCGTTTTGCAACCATTACTTCTTGCAAATG
CTAGCTCGGATAGGGAATGGTTACTATGACGGCACAAACAACACAGGTAGATTGTAATCT
CCTTTGGTCTATTTGTTTGATTATCGCTGATAGTGTTACTAATTCATAATCAAGAAACTA
AAAACATATTTCTTGGCTTTGTATTAACAATACACCTAGCCAGATTCATTCGAGCATCAG
ATGAGTAGACTATTTGAGATAGCTTCTTCAACGATCGTAGCCAATACTACTTTTGATGCT
CTCAAACTTCTCCGATCAGTTGAGGTAAC

Last Updated on 06.12.2018 16:11