Sequences from confirmation amplicon


Confirmation sequences for line 380E06 and hit At2g31320 are shown.
Line ID 380E06
Locus At2g31320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13356361
Primers used for PCR CN01 - 8474, show primer details
   
Sequence Name 17-K023835-022-380-E06C-8409
GenBank Accession LN493691 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K023835-022-380-E06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACAATGAAAAGATTG
GTGGTAACAAAGTGGAGGAAATGTCAAAGTCTGATGCGGTTCACGAATTCAAACGTCTAT
TTCTTGAAAAAACCGGAAACACATGGGAATCTTGGGAACAAAAAACGAATTTCCAGAAAC
AACCTGGAAAATTTCTCCCGTTGGACATTGTAAGCATCCATATGACTATATCTTTGTCTC
TTAAGATTCTCTTATGTATCGTTCAATAAACTCTTTTGTATCGTTTGTTTTAGGATTATG
GAGTTAATAAGCAAGTAGCCAAAAAAGAGCCATTTCAGACCAGTAGCAACCTTGCTCCAT
CATTAATAGAATTGATGAAGATGCTTTTTGATGTGGAAACTTACAGGTTAGAAACTTATT
TGCCATTGTCTTCTTCTCCTCT
   
Sequence Name 21-K023835-022-380-E06C-CN01
GenBank Accession LN495497 [GenBank]
Sequencing primer gene specific primer, CN01: 5'-AGGGAGAGAAGAGGAGAAGAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K023835-022-380-E06C-CN01
GGCAAATAAGTTTCTAACCTGTAAGTTTCCACATCAAAAAGCATCTTCATCAATTCTATT
AATGATGGAGCAAGGTTGCTACTGGTCTGAAATGGCTCTTTTTTGGCTACTTGCTTATTA
ACTCCATAATCCTAAAACAAACGATACAAAAGAGTTTATTGAACGATACATAAGAGAATC
TTAAGAGACAAAGATATAGTCATATGGATGCTTACAATGTCCAACGGGAGAAATTTTCCA
GGTTGTTTCTGGAAATTCGTTTTTTGTTCCCAAGATTCCCATGTGTTTCCGGTTTTTTCA
AGAAATAGACGTTTGAATTCGTGAACCGCATCAGACTTTGACATTTCCTCCACTTTGTTA
CCACCAATCTTTTCATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11