Sequences from confirmation amplicon


Confirmation sequences for line 381D03 and hit At3g05590 are shown.
Line ID 381D03
Locus At3g05590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1622823
Primers used for PCR 15lu - 8474, show primer details
   
Sequence Name 04-K114086-0022-381-D03C-15lu
GenBank Accession LN488003 [GenBank]
Sequencing primer gene specific primer, 15lu: 5'-CGTTGAAACCCTCTCTCTCTACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114086-0022-381-D03C-15lu
TTGATCTGTTACTTTCCTAGATACAGTGTCCAGTTTATGAATCTGCTAATGTGAACTTAG
TCTGTAACATGGCGGTGTTCGATAATTGATTCAAAATTCTCTACATTGTCTATGCTTTGG
ATTTTGAATTTTGCCTCTTGTTCTGATATTATAGGAAGATAAGATTGCCGTCTTGGTTGG
AACTATAACTGATGATTTGAGGGTACACGAGATTCCAGCCATGAAAGTGACTGCCTTGAG
GTTCACAGAGAGAGCAAGGGCTCGCATTGAGAAAGCTGGAGGTGAATGCTTAACCTTTGA
CCAGCTCGCTCTCAGAGCTCCATTGGGCCAGAACACGGTATTTCTCTTATCCAATCTGCT
ATTCTCCATTTTCAAGTTGTTAAGGATACTATATTTAATTTGATAGTTGTGTGTTCTTGT
GTTATAGGTTCTTCTTAGAGGACCTAAGAATTCACGTGAAGCAGTGAAGCATTTCGGACC
TGCTCCTGGTGTGCCACACAGTCACTCCAAGCCATATGTTCGGGCCAAGGGAAGGAAGTT
CGAGAAGGCCAGAGGAAAGAGGAAGAGTCGTGGATTCAAGGTTTAAGAAAGTTGTTGCCG
CCATTGGACTTGATTCTGGAGTACTGTTGCTTCTTGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K114085-0022-381-D03C-8409
GenBank Accession LN498941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114085-0022-381-D03C-8409
GCCAGAAGCACAGTACTCCAGAATCAAGTCCAATGGCGGCAACAACTTTCTTAAACCTTG
AATCCACGACTCTTCCTCTTTCCTCTGGCCTTCTCGAACTTCCTTCCCTTGGCCCGAACA
TATGGCTTGGAGTGACTGTGTGGCACACCAGGAGCAGGTCCGAAATGCTTCACTGCTTCA
CGTGAATTCTTAGGTCCTCTAAGAAGAACCTATAACACAAGAACACACAACTATCAAATT
AAATATAGTATCCTTAACAACTTGAAAATGGAGAATAGCAGATTGGATAAGAGAAATACC
GTGTTCTGGCCCAATGGAGCTCTGAGAGCGAGCTGGTCAAAGGTTAAGCATTCACCTCCA
GCTTTCTCAATGCGAGCCCTTGCTCTCTCTGTGAACCTCAAGGCAGTCACTTTCATGGCT
GGAATCTCGTGTACCCTCAAATCATCAGTTATAGTTCCAACCAAGACGGCAATCTTATCT
TCCTATAATATCAGAACAAGAGGCAAAATTCAAAATCCAAAGCATAGACNATGTAGAGAA
TTTTGAATCAATTATCGAACACCGCCATGTTACAGACTAAGTTCACATTAGCAGATTCAT
AAACTGGACACTGTATCTAGGAAATGTAACAGATTCAACATGACCTAAAACTAATTTAAC
CATAAAGATACAGTAGAGAGAGAGGTTT

Last Updated on 06.12.2018 16:11