Sequences from confirmation amplicon


Confirmation sequences for line 381D11 and hit At1g65190 are shown.
Line ID 381D11
Locus At1g65190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24216687
Primers used for PCR BW82 - 8474, show primer details
   
Sequence Name 26-K106020-0022-381-D11C-BW82
GenBank Accession LN497933 [GenBank]
Sequencing primer gene specific primer, BW82: 5'-GAGAGAGGAGCCAAGTTGTTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106020-0022-381-D11C-BW82
TCAAATTCTTCTCTGCTGATGAGATCCGCAAAGCCACCAACATTTTCAGCCACTCTAATC
TTGTTCATCAGGAAGAATTTTACTGCCAATGGTATTCAGGTAAGAACGAGAACCATCCCA
TGATACTCATCAGGAAAGATTCTAATGTGAGGGGAGGAGATCTTGTGTGCCGCAACATAG
CAGTTTCATCGATGGTGAGTGGTCACAAGAACTTTATGAAATTGGTTGGATGTTGTCTTG
AGTTGAAATATCCAGTCACGGTCTATCATGGTGTTAAGAAACATTATGGATTAGAAATAG
ATGAGAAGCCGTGGAAAAGAAGAATGAAGATAGCAGAAGATATCGCCACTGCTTTAGCTT
ACCTTCACACTGCCTTCCCTAGGCCCCTCGTATATATGATTTTGTCTCATCGGAATATCT
TATAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTA
   
Sequence Name 26-K106021-0022-381-D11C-8409
GenBank Accession LN497934 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106021-0022-381-D11C-8409
ATTTACATTGAATTATAAGATATTCCGATGAGACAAAATCATATACACTAAGGGCCTAGG
GAAGGCAGTGTGAAGGTAAGCTAAAGCAGTGGCGATATCTTCTGCTATCTTCATTCTTCT
TTTCCACGGCTTCTCATCTATTTCTAATCCATAATGTTTCTTAACACCATGATAGACCGT
GACTGGATATTTCAACTCAAGACAACATCCAACCAATTTCATAAAGTTCTTGTGACCACT
CACCATCGATGAAACTGCTATGTTGCGGCACACAAGATCTCCTCCCCTCACATTAGAATC
TTTCCTGATGAGTATCATGGGATGGTTCTCGTTCTTACCTGAATACCATTGGCAGTAAAA
TTCTTCCTGATGAACAAGATTAGAGTGGCTGAAAATGTTGGTGGCTTTGCGGATCTCATC
AGCAGAGAAGAATTTGATGGGATTGGATTTGCCATCGCAACATTCGATGAGCTCTTCTAA
CAACTTGGCTCCTCTCT

Last Updated on 06.12.2018 16:11