Sequences from confirmation amplicon


Confirmation sequences for line 381E02 and hit At1g29970 are shown.
Line ID 381E02
Locus At1g29970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10500077
Primers used for PCR AG06 - 8474, show primer details
   
Sequence Name 80-K105397-0022-381-E02C-AG06
GenBank Accession LN513955 [GenBank]
Sequencing primer gene specific primer, AG06: 5'-TTAAGTGACAGTTCGTTGTGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K105397-0022-381-E02C-AG06
TGGATCTCTTTGTTTCGAGTGAGATTCTAGATGAACTGATTGCCTCTTGGAATCAATATA
AAAAGTCTGTGTAAAACCTTCTTGAGGCTTGTCTCTATCCACTAATGTTTAGATTTACAA
GAGCATCAAGATTAGCTAATATTCGATAAATGGATTTGTTGGTCATTCAATCTGGATATA
CTTTGATGAACTCCCTGACACGGAGATACAAGTGCAATCCGCCTTATATGATTCTGTCGT
TTAACGAGAGAAAGCTGAAAGCATACATCTTGTGACTTGAAACTCGTTCCAGTTGCTAAT
TAAAGTTTTTGTTGTCTATGGTCCCAGGTTTATCATGGGCTTCTTGTTCGGTGGGATCCC
TTGGTACCTCGGTGCTTTTATTGTTCTAGTCACATCTGTTGATCACCGCGAGAAAGCTGG
TTACGTTGCCTGTTCAATCGCAGTGAGTTCCTCTCTCTCATTACATTGTAGATTTCTCGT
TGCCTGTTTCTCTCTCATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 72-K105398-0022-381-E02C-8409
GenBank Accession LN512725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K105398-0022-381-E02C-8409
ATTTACATTGAATAATCCTGCAATGAGAGAGAAACAGGCAACGACCATTCTACAATGTAA
TGAGAGAGAGGAACTCACTGCGATTGAACAGGCAACGTAACCAGCTTTCTCGCGGTGATC
AACAGATGTGACTAGAACAATAAAAGCACCGAGGTACCAAGGGATCCCACCGAACAAGAA
GCCCATGATAAACCTGGGACCATAGACAACAAAAACTTTAATTAGCAACTGGAACGAGTT
TCAAGTCACAAGATGTATGCTTTCAGCTTTCTCTCGTTAAACGACAGAATCATATAAGGC
GGATTGCACTTGTATCTCCGTGTCAGGGAGTTCATCAAAGTATATCCAGATTGAATGACC
AACAAATCCATTTATCGAATATTAGCTAATCTTGATGCTCTTGTAAATCTAAACATTAGT
GGATAGAGACAAGCCTCAAGAAGGTTTTACACAGACTTTTTATATTGATTCCAAGAGGCA
ATCAGTTCATCTAGAATCTCACTCGAAACAAAGAGATTCCAGGAAAGAAGACTCTGTTAC
AGAATCTAGAGCTAGTTCCACAACGAACTGTCACTTA
Primers used for PCR AG07 - 8474, show primer details
   
Sequence Name 57-K118809-0022-381-E02C-AG07
GenBank Accession LN509555 [GenBank]
Sequencing primer gene specific primer, AG07: 5'-GAACTTTTTGTGTAGCAATGGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118809-0022-381-E02C-AG07
ATAGATATGCCACCAAGGGAATCACCACATATATACAATATTTAGTTATGATATAAAATT
CAACACAAAGTTACAAATATTTCCCTTCTGTAACTCACTAAAATCAGCAATAACTGGTGT
AAATGATATTGATATATATCTACGTTTTCGAGTATATATGTAGATATATATACAAGATTG
AGTTTGGTTTTGGCTGGGGAGTAGTTTCACCAGATGTTAATATCCCCAGTCATCCCGAGC
ATGACGGCAATCAAATAGACAACGGACTGGAAGAATTCATAAAAAGAAAAAGAAATTATG
AGAGACAATGATGAATATAGATGAGTGACCAACAATGTAAATGGATCAACAATGTAAAGA
AAGAGTAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 25-K118808-0022-381-E02C-8409
GenBank Accession LN497676 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118808-0022-381-E02C-8409
TCATTGAATTACTCTTTCTTTACTTGTTGATCCATTTACATTGTTGGTCACTCATCTATA
TTCATCATTGTCTCTCATAATTTCTTTTTCTTTTTATGAATTCTTCCAGTCCGTTGTCTA
TTTGATTGCCGTCATGCTCGGGATGACTGGGGATATTAACATCTGGTGAAACTACTCCCC
AGCCAAAACCAAACTCAATCTTGTATATATATCTACATATATACTCGAAAACGTAGATAT
ATATCAATATCATTTACACCAGTTATTGCTGATTTTAGTGAGTTACAGAAGGGAAATATT
TGTAACTTTGTGTTGAATTTTATATCATAACTAAATATTGTATATATGTGGTTGATTCCC
TTGGTGGCATATCTATCTATATGTAAAATATTTTGTGATTTGTTTTGACCATTGCTACCA
NAAAAGTT

Last Updated on 06.12.2018 16:11