Sequences from confirmation amplicon


Confirmation sequences for line 381H02 and hit At5g12370 are shown.
Line ID 381H02
Locus At5g12370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4007329
Primers used for PCR 10um - 8474, show primer details
   
Sequence Name 43-K117317-0022-381-H02C-10um
GenBank Accession LN504982 [GenBank]
Sequencing primer gene specific primer, 10um: 5'-GTCGTATTTCAAGTGTTGGACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117317-0022-381-H02C-10um
GTAATTACCTTGCTTATAACCAACTATCTGGTGGTCTATCCAATATTAACCCATGCTTCC
TTTGTCAGAGTGCAGATGCTCAGCGGGAAACTGCTAGCCAGACGATAGATCTTATAAAGG
TAAGTGTGCATCTATGTTGCAAGGTTGGTCACATATTTAGTTAGATGAATGCTTAAAGTA
ATCCTTGTTCAACAAATGCAGTACCTAATGGAGTTCAACGGCAGCCCAGGGGATCTTATG
GAGCTTTCCGCTTTGTTTTCTGATGATAGCCGCGTAGCTGAGGCTGCTTCTATCGCTCAG
AAATTACGTATGATGTGATAGCTTTATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 34-K117316-0022-381-H02C-8409
GenBank Accession LN501573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117316-0022-381-H02C-8409
GAATATATCCTGATAAAGCTATCACATCATACGTAATTTCTGAGCGATAGAAGCAGCCTC
AGCTACGCGGCTATCATCAGAAAACAAAGCGGAAAGCTCCATAAGATCCCCTGGGCTGCC
GTTGAACTCCATTAGGTACTGCATTTGTTGAACAAGGATTACTTTAAGCATTCATCTAAC
TAAATATGTGACCAACCTTGCAACATAGATGCACACTTACCTTTATAAGATCTATCGTCT
GGCTAGCAGTTTCCCGCTGAGCATCTGCACTCTGACAAAGGAAGCATGGGTTAATATTGG
ATAGACCACCAGATAGTTGGTTATAAGCAAGGTAAATTTTACCTGCAAATGATCTCCTAT
TTTTGCAGCGGTCTGTCCAACACTTAAAATACGAC

Last Updated on 06.12.2018 16:11