Sequences from confirmation amplicon


Confirmation sequences for line 382E10 and hit At1g73680 are shown.
Line ID 382E10
Locus At1g73680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27706751
Primers used for PCR 26vf - 8474, show primer details
   
Sequence Name 54-K117939-0022-382-E10C-26vf
GenBank Accession LN508616 [GenBank]
Sequencing primer gene specific primer, 26vf: 5'-TTTCCAGTGTTATTAGGAGTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117939-0022-382-E10C-26vf
TCTGGTAATGTCGGTCCGATCAACGGTCAGGGGTACGACACCGATGAGTTCTGTTATCGT
ACGGCTGACGGCAAGTGTAACCATCCCTCCGATAACACCATCGGCAGCCAAGGCAGCTTC
ATCGGCCGGAATATGCCTCCCTCTACTTCTCAGTACGGCGTAAGTTTTTTTCTTGCTCTC
CTTCATATTCACAATCACTTTTTCACAACATGCAAATTAATTTTGTACTTTTGATTAAAT
CTATAAAATAAAGCGAAGTGATATATAATATAGCAAAAGTAAATAGTAGTGAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 45-K117938-0022-382-E10C-8409
GenBank Accession LN505674 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117938-0022-382-E10C-8409
GCCATTTCCTACTATTTACTTTTGCTATATATATATCGCTTCGCTTTATTTTATAGATTT
ACTCGAAAGTACAAAATTAATTTGCATGTTGTGAAAAAGTGATTGTGAATATGAGGGAGA
GCAGGAAAAAAACTTACGCCGAACTGAGAAGTACAGGGAGGCATATTCCGGCCGATGTAA
TCTGCCGTGGCTGCCAAAGGTGCTATCGCATGGATGGTTACACTTGCCGACATTCGTACG
ATAGCAGAACTCATCGGTGTCGTGCCCAGGGCTGTTTATCAGACCGAAATTTAATAGAAT
GGAACGGGCATCTTGACGGGGACATCTCCCCGGATCAGCCACGCCTATGACGGTCAAAAA
GGACAAAAAGAAAGAGTAATTCCCATTTTTTTTTCACTTCAAAAATG

Last Updated on 06.12.2018 16:11