Sequences from confirmation amplicon


Confirmation sequences for line 382F01 and hit At2g31320 are shown.
Line ID 382F01
Locus At2g31320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13356988
Primers used for PCR DN24 - 8474, show primer details
   
Sequence Name 18-K101829-0022-382-F01C-8409
GenBank Accession LN494245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101829-0022-382-F01C-8409
AAATGATCCCTCAAGCAATGAAGTGCAAAGATAGTCTACAATATAATTTTTGACAGAAGA
ACATACCCTTTCCTTAGTATTTCCAGCAATTGAAACTTTAAGATCTGCTAGCCTTTCACT
CCTTGAGGAGTCAGTTGCATCTTTAGAACCTTGACCCTGAGATGTCTCGCCAGACAATAC
AGGACGCAGAATACGTTTTGGCTTCACACTCTTTTGAGACTTATTCCACTGCAAGTAATC
AAAATATAAATATTAACTTCATGAGTAATATATACCACATCTAACCTAACCCATACCAAT
ATCAACTCTACAAAAAGACTGGTTTACCTTCAGAAGGAATTGATTTTCTGTTTCGTCAGG
GATTTTCCACTTCCCTTTGATGCGGTCTGGATCCAAAGTGGAATGAGAACATTTGCTCCA
TTCTGAGATGTATCCATGGCATCGGTAAAGTCCTCCGGAGAAAGAAAGATGCCCAGAGCA
CATTGGGCAGAGAGCGAGTGGGCCAAACATCATGCCATCACNAACTAAAT
   
Sequence Name 62-K101832-0022-382-F01C-DN24
GenBank Accession LN510718 [GenBank]
Sequencing primer gene specific primer, DN24: 5'-TATTTAGTGCTGATGGCATGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K101832-0022-382-F01C-DN24
GGCATCTTTCTTTCTCCGGAGGACTTTACCGATGCCATGGATACATCTCAGAATGGAGCA
AATGTTCTCATTCCACTTTGGATCCAGACCGCATCAAAGGGAAGTGGAAAATCCCTGACG
AAACAGAAAATCAATTCCTTCTGAAGGTAAACCAGTCTTTTTGTAGAGTTGATATTGGTA
TGGGTTAGGTTAGATGTGGTATATATTACTCATGAAGTTAATATTTATATTTTGATTACT
TGCAGTGGAATAAGTCTCAAAAGAGTGTGAAGCCAAAACGTATTCTGCGTCCTGTATTGT
CTGGCGAGACATCTCAGGGTCAAGGTTCTAAAGATGCAACTGACTCCTCAAGGAGTGAAA
GGCTAGCAGATCTTAAAGTTTCAATTGCTGGAAATACTAAGGAAAGGGTATGTTCTTCTG
TCAAAAATTATATTGTAGACTATCTTTGCACTTCATTGCTTGATGGGATCATTTTGCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11