Sequences from confirmation amplicon


Confirmation sequences for line 382G07 and hit At1g17590 are shown.
Line ID 382G07
Locus At1g17590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6052422
Primers used for PCR 44vk - 8474, show primer details
   
Sequence Name 71-K117751-0022-382-G07C-44vk
GenBank Accession LN512625 [GenBank]
Sequencing primer gene specific primer, 44vk: 5'-ACTCAGTGATTCAGTCACACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K117751-0022-382-G07C-44vk
GGTGGAGTTGAATGTGCCACAGAGCTAGTAAATGAAACTTTCTTATCCATGTGACTCAGA
GGCAACAATTAGCTCTCTCTCTCTCTGCTTCAACAGAGAAGATTGATGATACATGTGTCT
GTCAAAGAAGCTCCAAATTCAGAACATCTTTATAAATGATAAAACTTTCAAAAGATATTT
ATTCCTGGAAACAGCAAAACCTTTGACGATGTAGTAGACAAAGAAGGGGATAATCCAAAA
AGGGAAAAAAACCCAGAAACAGAGCAACATTCAGAACTATTTCGGGCAAACTATTAGAAG
AGCGCGGCAGAAAAAAAAGTAAAGTTCCGACCAACTAAAATCAGCACCATTACTTTCGTC
CTAAAAGGCGTTGACTTGGGAAGAAATTTCCAGAAGATTTCCGAATCAGCACATCACTCT
AAAAAAAAGCGAAGAAATGACGGATTCAAAGAGAAGGAGATAGAGAGACCTTACGGATTC
AAGGAAGAAAGAGTAATCGAAGCCATTTTCGACGGCAGCGGCGGCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 46-K117750-0022-382-G07C-8409
GenBank Accession LN505995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117750-0022-382-G07C-8409
CCGCCGCTGCCGTCGAAAATGGCTTCGATTACTCTTTCTTCCTTGAATCCGTAAGGTCTC
TCTATCTCCTTCTCTTTGAATCCGTCATTTCTTCGCTTTTTTTTAGAGTGATGTGCTGAT
TCGGAAATCTTCTGGAAATTTCTTCCCAAGTCAACGCCTTTTAGGACGAAAGTAATGGTG
CTGATTTTAGTTGGTCGGAACTTTACTTTTTTTTCTGCCGCGCTCTTCTAATAGTTTGCC
CGAAATAGTTCTGAATGTTGCTCTGTTTCTGGGTTTTTTTCCCTTTTTGGATTATCCCCT
TCTTTGTCTACTACATCGTCAAAGGTTTTGCTGTTTCCAGGAATAAATATCTTTTGAAAG
TTTTATCATTTATAAAGATGTTCTGAATTTGGAGCTTCTTTGACAGACACATGTATCATC
AATCTTCTCTGTTGAAGCAGAGAGAGAGAGAGCTAATTGTTGCCTCTGAGTCACATGGAT
AAGAAAGTTTCATTTACTAGCTCTGTGGCACATTCAACTCCACCATACCTTAGTACTTCC
ATCTCAGGGGGACTTCCAACCAAATCCAATGGTGTGATGTCCCCCC

Last Updated on 06.12.2018 16:11