Sequences from confirmation amplicon


Confirmation sequences for line 383H03 and hit At1g12730 are shown.
Line ID 383H03
Locus At1g12730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4337343
Primers used for PCR 14ad - 8474, show primer details
   
Sequence Name 58-K106210-0022-383-H03C-14ad
GenBank Accession LN509713 [GenBank]
Sequencing primer gene specific primer, 14ad: 5'-ACCATCTTTAATGTTCCGGTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K106210-0022-383-H03C-14AD
TTTGCTATTAGCCTTCACTTCGATTCTTAAATCTTATCCTTCAGTAAGTAATTTAACTTC
ACTATGAAACAATATTGTATCTTTCTTGAAAAAGCTTTCTGTTCATAGAATCTTAAATCT
TGAACGTATTCCTTACTCTCATCACTAGGTTGGAGATGCAGCTTTATACTTGAGTTTATG
GGCATTGTTTGTCAATGAATTAACAGGTAGTGAAACAAATTCCATCTCATCTCTTTCAGT
ATGATCTTGAACCGTCATGAACTTTTCACATCTAACTGGGCTTCTGTGTAAACGTTAGAC
ATGGAGTACTCGTTCTTTATCTTCTGTGGATATATCGGATTTTCGCTTCTCAGCCCTGTG
ATGCACAATTTCTGGATATGGAGGGTAAAGCTTCAAACTCAACTCCATTCAATCACAATA
AAACTTAAAAGAAACCAGAACCCATTTGATATTTTTGGATTTACAGGGAACTGGAAATGC
AAACTTCTACTTCTCCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCC
   
Sequence Name 74-K106211-0022-383-H03C-8409
GenBank Accession LN513078 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K106211-0022-383-H03C-8409
GAGCCATTTACAATTGAATATGGAGAAGTAGAAGTTTGCATTTCCAGTTCCCTGTAAATC
CAAAAATATCAAATGGGTTCTGGTTTCTTTTAAGTTTTATTGTGATTGAATGGAGTTGAG
TTTGAAGCTTTACCCTCCATATCCAGAAATTGTGCATCACAGGGCTGAGAAGCGAAAATC
CGATATATCCACAGAAGATAAAGAACGAGTACTCCATGTCTAACGTTTACACAGAAGCCC
AGTTAGATGTGAAAAGTTCATGACGGTTCAAGATCATACTGAAAGAGATGAGATGGAATT
TGTTTCACTACCTGTTAATTCATTGACAAACAATGCCCATAAACTCAAGTATAAAGCTGC
ATCTCCAACCTAGTGATGAGAGTAAGGAATACGTTCAAGATTTAAGATTCTATGAACAGA
AAGCTTTTTCAAGAAAGATACAATATTGTTTCATAGTGAAGTTAAATTACTTACTGAAGG
ATAAGATTTAAGAATCGAAGTGAAGGCTAAATAAGCGAAAGCAAGGAAGCAGGGTCGATG
TTTTAACCGGAACTAAAAAGATGG

Last Updated on 06.12.2018 16:11