Sequences from confirmation amplicon


Confirmation sequences for line 384G06 and hit At3g44990 are shown.
Line ID 384G06
Locus At3g44990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16448336
Primers used for PCR 27fb - 8474, show primer details
   
Sequence Name 59-K111269-0022-384-G06C-27fb
GenBank Accession LN510032 [GenBank]
Sequencing primer gene specific primer, 27fb: 5'-CTAGCTCTCTTAGTGTTGTGCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111269-0022-384-G06C-27fb
GGGATACTACCCGAGTTCCCGAAGTACCGACTTCACCTTTTGATCGTGAATTCCGTACTT
TATGGGGCTCTCAACACCAACGTAGAGAGCAAGACGTTGTCACTCTTTGGCTCGACAAAT
CCACTGGTCTTTTTCTTTCTTTACTTTTTTCGTTTTCAACCAAATTTGAGTCCATGTTAT
AAAATGCACTAAGAGATTATATGCTATTCATTATGAACAGACCATTAAAATTAATGAATG
TACTGATTAGAATCAATTTAGTAAGTGGTGATAACTTTGTCTGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTATAA
   
Sequence Name 43-K111268-0022-384-G06C-8409
GenBank Accession LN504876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111268-0022-384-G06C-8409
CACTTACTAAATTGATTCTAATCAGTACATTCATTAATTTTAATGGTCTGTTCATAATGA
ATAGCATATAATCTCTTAGTGCATTTTATAACATGGACTCAAATTTGGTTGAAAACGAAA
AAAGTAAAGAAAGAAAAAGACCAGTGGATTTGTCGAGCCAAAGAGTGACAACGTCTTGCT
CTCTACGTTGGTGTTGAGAGCCCCATAAAGTACGGAATTCACGATCAAAAGGTGAAGTCG
GTACTCGGGAACTCGGGTAGTATCCCGGTGAAGGAGAACGTTGGCTGTGACCACTACTAG
GGCACAACACTAAGAGAGCTAGAC

Last Updated on 06.12.2018 16:11