Sequences from confirmation amplicon


Confirmation sequences for line 384G12 and hit At5g64290 are shown.
Line ID 384G12
Locus At5g64290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25716460
Primers used for PCR SD15 - 8474, show primer details
   
Sequence Name 03-K103490-0022-384-G12C-SD15
GenBank Accession LN487452 [GenBank]
Sequencing primer gene specific primer, SD15: 5'-GAGCGAAGAAGAAAGAGATAACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103490-0022-384-G12C-SD15
TGAGAAGCGGCGGAGAAGAAAGCGTTTTGGTGACGATTGAAGCGGTGAGACCAATGAAAG
CCCAAGCTCCGACTGGGAGAGGACTAAGGACGAGACCAGCGATTGTTGAGAGGAAGATTG
AGAGTAATTGCCAGCCTTGTGGTGTGACTCCTTCTGGTACAGGAACAGCGAATCGGAGGA
TTAGGCCGACGGAGATTGAGAGAATCAGAGGGATGAGTTTCGCTCCTTGAGGAGACGGAG
ACGGAGACGGAGATGGGGGAGGAGGAGGAGGAGCGTTGTCTTGCGGTGCGGCGGCGATTC
GTGTGGAGAATCGAGGAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 27-K103491-0022-384-G12C-8409
GenBank Accession LN498348 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103491-0022-384-G12C-8409
AGCCATTTAAATTCCTCGATTCTCCACACGAATCGCCGCCGCACCGCAAGACAACGCTCC
TCCTCCTCCTCCCCCATCTCCGTCTCCGTCTCCGTCTCCTCAAGGAGCGAAACTCATCCC
TCTGATTCTCTCAATCTCCGTCGGCCTAATCCTCCGATTCGCTGTTCCTGTACCAGAAGG
AGTCACACCACAAGGCTGGCAATTACTCTCAATCTTCCTCTCAACAATCGCTGGTCTCGT
CCTTAGTCCTCTCCCAGTCGGAGCTTGGGCTTTCATTGGTCTCACCGCTTCAATCGTCAC
CAAAACGCTTTCTTTCTCCGCCGCTTTCTCAGCTTTCACAAGCGAGGTTATCTGGTTGAT
CGTTATCTCTTTCTTCTTCGCT

Last Updated on 06.12.2018 16:11