Sequences from confirmation amplicon


Confirmation sequences for line 385H03 and hit At2g27970 are shown.
Line ID 385H03
Locus At2g27970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11912562
Primers used for PCR MK17 - 8474, show primer details
   
Sequence Name 73-K030758-022-385-H03C-MK17
GenBank Accession LN512821 [GenBank]
Sequencing primer gene specific primer, MK17: 5'-GAGTTTCTTTGAATCAGTATCGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K030758-022-385-H03C-MK17
ATGATACCTTGTTTGTTGTTCATAGTTATGGACTTAAGACTCTAAGAGGAGCAATAAAAA
GAGGTTGAAATGATGAAACAAGCACATAAGCATGAGAAATAACGTCTTCATTCTTAACCA
TGAAGAAGTTCAAACGTGGATCCAACCAAAGTCTAGCAAAGGACCAGACTTAACATAACA
TTAAAAGTTGTTCTACCAAACTCACATGATTCTCCACTGAAGAGAATTCATGAACACAAT
AAAAAAAACTACAGGAACAAAACATCAGAGTAGTATTTTAAAACCTCGTTTACGAGAGAC
ATAAACAGAGATTAGGGAGAATTTTGCATAATTCTTCTACTTAGCAATCTGAGCTTGGTG
CTCCTGCTGGTAGTTAAGAGGCCTCCTAAAGAGCATGATATGTGGCTCAGGACGATGAAT
CGCGTAATGAACCCATCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATT
   
Sequence Name 34-K030758-022-385-H03C-8409
GenBank Accession LN501297 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K030758-022-385-H03C-8409
TTTACAATTGGATGGGTTCATTACGCGATTCATCGTCCTGAGCCACATATCATGCTCTTT
AGGAGGCCTCTTAACTACCAGCAGGAGCACCAAGCTCAGATTGCTAAGTAGAAGAATTAT
GCAAAATTCTCCCTAATCTCTGTTTATGTCTCTCGTAAACGAGGTTTTAAAATACTACTC
TGATGTTTTGTTCCTGTAGTTTTTTTTATTGTGTTCATGAATTCTCTTCAGTGGAGAATC
ATGTGAGTTTGGTAGAACAACTTTTAATGTTATGTTAAGTCTGGTCCTTTGCTAGACTTT
GGTTGGATCCACGTTTGAACTTCTTCATGGTTAAGAATGAAGACGTTATTTCTCATGCTT
ATGTGCTTGTTTCATCATTTCAACCTCTTTTTATTGCTCCTCTTAGAGTCTTAAGTCCAT
AACTATGAACAACAAACAAGGTATCATTACAATGCCAATCTGAAGGTCTAATCACGATTC
TCATGGCGATACTGATTCAAGGGAAACTC

Last Updated on 06.12.2018 16:11