Sequences from confirmation amplicon


Confirmation sequences for line 386C01 and hit At2g26760 are shown.
Line ID 386C01
Locus At2g26760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11402008
Primers used for PCR MZ55 - 8474, show primer details
   
Sequence Name 09-K025300-022-386-C01C-8409
GenBank Accession LN490018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K025300-022-386-C01C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACAATATACATACTTTATG
AGTTGCAATATTGGATGAAAACAGAAAATGATCAGAGATGTTTAAAATTACCTTTCCTGT
TGCAACATCACGACCAGTGACAAGATTACCAATGTCTCCAAGAACTTTCCGGTTTTGTCT
TCCATGTCCTGCAACATTCTTCGGTTTGATTTCACCTAACAAAATTCATTTATAACACAA
ACCCATCAGTAATTAACCTAATAGAAACTTGCAGTCTCATCAGATCAGATCCAATCATGA
ACATTCAATTGCG
   
Sequence Name 10-K025300-022-386-C01C-MZ55
GenBank Accession LN490491 [GenBank]
Sequencing primer gene specific primer, MZ55: 5'-CAATTTCGTGGATTTTGTTTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K025300-022-386-C01C-MZ55
GATTGAAATTGAAATGTACCTTGTTTAAATTCGTTATAGATCTTGATAGATTTATCTGTT
GTATCCGCAATTGAATGTTCATGATTGGATCTGATCTGATGAGACTGCAAGTTTCTATTA
GGTTAATTACTGATGGGTTTGTGTTATAAATGAATTTTGTTAGGTGAAATCAAACCGAAG
AATGTTGCAGGACATGGAAGACAAAACCGGAAAGTTCTTGGAGACATTGGTAATCTTGTC
ACTGGTCGTGATGTTGCAACAGGAAAGGTAATTTTAAACATCTCTGATCATTTTCTGTTT
TCATCCAATATTGCAACTCATAAAGTATGTATATTGTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11