Sequences from confirmation amplicon


Confirmation sequences for line 386D12 and hit At3g20410 are shown.
Line ID 386D12
Locus At3g20410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7117039
Primers used for PCR TR04 - 8474, show primer details
   
Sequence Name 03-K026133-022-386-D12C-8409
GenBank Accession LN487328 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K026133-022-386-D12C-8409
GCTTCAAGTCTCTATGCAACACACCCATGAAATGACAAATCTTGACAACATTCACAATCT
GTCTACAAACAGAAGCAGCAGCTCTCTCAGTGTAATGTCCCTTAGCAATGATTCTATCAA
ACAATTCACCACCAGCACAAAGCTCCATCACCAAATTCACAGCTTTCTCATCCTCATAAG
CTCCTTTAAACTCCACAATATTAGGCTGCCCACTCAAATGCTGCATTATCTGAATCTCTC
TCCTCATATCATCCTTATCAGCTTTAGTCACCAGCTTCTTCTTCGAGATCGATTTGCAAG
CGTACTTCTTCCCCGTGGAATTCTCTGTGCACAGATACGTTACCCCAAATTGACCACGAC
CTAGCTCTTTACCCAATGTGTAAAAGAGCTTCACGTCTTCGAAAGCGTTCTCTAGTATTG
AATTGCTCTTAGTGGTGGTCT
   
Sequence Name 04-K026133-022-386-D12C-TR04
GenBank Accession LN487768 [GenBank]
Sequencing primer gene specific primer, TR04: 5'-CTAAGTCCCAAGACCACCACTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K026133-022-386-D12C-TR04
ATTCAATACTAGAGAACGCTTTCTAAGACGTGAAGCTCTTTTACACATTGGGTAAAGAGC
TAGGTCGTGGTCAATTTGGGGTAACGTATCTGTGCACAGAGAATTCCACGGGGAAGAAGT
ACGCTTGCAAATCGATCTCGAAGAAGAAGCTGGTGACTAAAGCTGATAAGGATGATATGA
GGAGAGAGATTCAGATAATGCAGCATTTGAGTGGGCAGCCTAATATTGTGGAGTTTAAAG
GAGCTTATGAGGATGAGAAAGCTGTGAATTTGGTGATGGAGCTTTGTGCTGGTGGTGAAT
TGTTTGATAGAATCATTGCTAAGGGACATTACACTGAGAGAGCTGCTGCTTCTGTTTGTA
GACAGATTGTGAATGTTGTCAAGATTTGTCATTTCATGGGTGTGTTGCATAGAGACTTGA
AGCTACATGGATCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11