Sequences from confirmation amplicon


Confirmation sequences for line 386G06 and hit At1g29060 are shown.
Line ID 386G06
Locus At1g29060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10148020
Primers used for PCR 09jr - 8474, show primer details
   
Sequence Name 34-K113214-0022-386-G06C-8409
GenBank Accession LN501483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113214-0022-386-G06C-8409
TTTCATTGAATTATCCTGATCCATTTACAATTGCTGATCCATCTTTGGTGTGTGATTTGT
AGAGAAGGGCTAAGCACTAGAAATGCTTCAGGTTCAGAGGAAATTCAGCTGAGGATTGAT
CCTATGCACTCTGACCTGGATGATGAGATTCTAGGTCTCCATGGCCAAGTCAGGCAATTG
AAAAATGTAAGAAACCCTCTAAATCCCATTTCGATCTTTCTACTTTTTATCTATAGTGCC
ATTTGTATTGTCTTCTTGTGGTTCTGAAGGATCATTGTTCTCTTTAAGCTTTAAGTTGAT
CATATGAATTTGGAAATTTGTCTTCTTTTCGGTTTTGACAGTGGTTAGAGCTTGGTTAAT
TAGTTGTGTAGGTGTTTTTTTGTTCAGGGTACTATGATAATGTTATTGTTCACAATACTT
ATCAGTCAGTCCACGATTAATTAGTTGAATCCACTTGTCTGATGTCTTTTGTATACATCT
GTGGTTATTGTTTTATATCACCACCCTTCTTGAAGGAATCAGTCGACATTCTCTACAATT
CCTTGTGTTTTACCTTGCACTTTTGCGCATTATTTATCCCTCATGATTTTTTGTTATATC
GTTGATCGCTTATTTTGATGCAGATTGCTCAA
   
Sequence Name 34-K113215-0022-386-G06C-09jr
GenBank Accession LN501484 [GenBank]
Sequencing primer gene specific primer, 09jr: 5'-CTTGAGCAATCTGCATCAAAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113215-0022-386-G06C-09jr
AAAATCTGAGGGATAAATATGCGCAAAGTGCAAGGTAAAACACAAGGAATTGTAGAGAAT
GTCGACTGATTCCTTCAAGAAGGGTGGTGATATAAAACAATAACCACAGATGTATACAAA
AGACATCAGACAAGTGGATTCAACTAATTAATCGTGGACTGACTGATAAGTATTGTGAAC
AATAACATTATCATAGTACCCTGAACAAAAAAACACCTACACAACTAATTAACCAAGCTC
TAACCACTGTCAAAACCGAAAAGAAGACAAATTTCCAAATTCATATGATCAACTTAAAGC
TTAAAGAGAACAATGATCCTTCAGAACCACAAGAAGACAATACAAATGGCACTATAGATA
AAAAGTAGAAAGATCGAAATGGGATTTAGAGGGTTTCTTACATTTTTCAATTGCCTGACT
TGGCCATGGAGACCTAGAATCTCATCATCCAGGTCAGAGTGCATAGGATCAATCCTCAGC
TGAATTTCCTCTGAACCTGAAGCATTTCTAGTGCTTAGCCCTTCTCTACAAATCACACAC
CAAAGATGGATCAGCAATTGTAAATGGATCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11