Sequences from confirmation amplicon


Confirmation sequences for line 386G07 and hit At4g13130 are shown.
Line ID 386G07
Locus At4g13130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7649291
Primers used for PCR 56qv - 8474, show primer details
   
Sequence Name 72-K115779-0022-386-G07C-56qv
GenBank Accession LN512782 [GenBank]
Sequencing primer gene specific primer, 56qv: 5'-GTATTATAATGGTTTCCTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K115779-0022-386-G07C-56qv
GGTCTCTTCCAATCAAGATTCATTAAACAAGACAATGTTCACAATCACACATTAACAAGC
TTTTCAAATCAGAGAAATTTCCAAGATTCAAAACTTACAATGAACATCAGAGTCTCTTTG
ATAACTCTACTCAAAACTATAGTCTCCTAAATTGGGATTTCATTCAAACATCTTTATTGT
CAATTTCAGCTTGCTTTCTTCTTCTTTCTTCTTCGTCCCATCTTAGTCGACCAAACAAAA
AACAATGAATGGTGCAATATACTAACTCAGACCACTGGATAACTGTGTTATGTGGACAAC
GACTCTTACATTGGAAGCAAATAGGTCGAGACATAACCTGAGTTTTTAAAAGAACATCTG
CATGCCTGTGGTCGTACTCAAACGATGAACCGGGCTTCAAGTATAATTCTTTCCCAATCA
AACATTGAATGTGCAGGGTGACACAACAATATTCATCACACTTATAAAACCGTTCTTCTG
AATTTATCTCTTTCTCGCAGGCTTCACACCAATACATCATGGTACTTGTCTCCTCCCCAT
AAGAAAGAGTGAGCATGTGCGTATCCATCATTTACTTTTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTT
ATT
   
Sequence Name 24-K115778-0022-386-G07C-8409
GenBank Accession LN497130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115778-0022-386-G07C-8409
TTGAATTATCCTGAAGAGTAAATGATGGATACGCACATGCTCACTCTTTCTTATGGGGAG
GAGACAAGTACCATGATGTATTGGTGTGAAGCCTGCGAGAAAGAGATAAATTCAGAAGAA
CGGTTTTATAAGTGTGATGAATATTGTTGTGTCACCCTGCACATTCAATGTTTGATTGGG
AAAGAATTATACTTGAAGCCCGGTTCATCGTTTGAGTACGACCACAGGCATGCAGATGTT
CTTTTAAAAACTCAGGTTATGTCTCGACCTATTTGCTTCCAATGTAAGAGTCGTTGTCCA
CATAACACAGTTATCCAGTGGTCTGAGTTAGTATATTGCACCATTCATTGTTTTTTGTTT
GGTCGACTAAGATGGGACGAAGAAGAAAGAAGAAGAAAGCAAGCTGAAATTGACAATAAA
GATGTTTGAATGAAATCCCAATTTAGGAGACTATAGTTTTGAGTAGAGTTATCAAAGAGA
CTCTGATGTTCATTGTAAGTTTTGAATCTTGGAAATTTCTCTGATTTGAAAAGCTTGTTA
ATGTGTGATTGTGAACATTGTCTTGTTTAATTGAATCTTTGATTTGTGAAGGAGACCGCT
AGACGAACTAGAACGGTACATTGGTAGGAAACCANTATAATA

Last Updated on 06.12.2018 16:11