Sequences from confirmation amplicon


Confirmation sequences for line 386H08 and hit At3g01340 are shown.
Line ID 386H08
Locus At3g01340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:127934
Primers used for PCR BM85 - 8474, show primer details
   
Sequence Name 14-K104415-0022-386-H08C-BM85
GenBank Accession LN492494 [GenBank]
Sequencing primer gene specific primer, BM85: 5'-TTCATGATGTGGTCATGGATTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K104415-0022-386-H08C-BM85
GATCAAGATAACCGGAGTAAGCAACAGCGGTGGATCTCAGCATCTAGCTACCTTAACCGG
TCATCGAGGTCCTGTCTGGCAAGTCGCTTGGGCGCACCCAAAGTTTGGTTCACTTCTCGC
TTCATGTTCCTATGATGGGCAAATTATATTATGGAAAGAAGGTAACCAAAACCAATGGAC
ACAAGCTCATGTCTTCACAGACCACAAAGTATCTGTCAACTCTATCGCTTGGGCTCCTCA
TGAACTTGGACTCTCCTTAGCTTGCGGAGCATCTGATGGAAACATATCAGTTTTCTCGGC
TCGAGCTGATGGCGGTTGGGACACGACAAAGATAGACCAAGCACACCCTGTTGGTGTCAC
TTCAGTGTCGTGGGCACCGGCCACAGAACCAGGCGCACTCGTTAGCTCTGGTATGATCGA
TCCGGTCTACATGGATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 30-K104416-0022-386-H08C-8409
GenBank Accession LN499705 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K104416-0022-386-H08C-8409
CATTTACAATTGAATATATATCCATGTAGACCGGATCGATCATACCAGAGCTAACGAGTG
CGCCTGGTTCTGTGGCCGGTGCCCACGACACTGAAGTGACACCAACAGGGTGTGCTTGGT
CTATCTTTGTCGTGTCCCAACCGCCATCAGCTCGAGCCGAGAAAACTGATATGTTTCCAT
CAAATGCTCCGCAAGCTAAGGAGAGTCCAAGTTCATGAGGAGCCCAAGCGATAGAGTTGA
CAGATACTTTGTGGTCTGTGAAGACATGAGCTTGTGTCCATTGGTTTTGGTTACCTTCTT
TCCATAATATAATTTGCCCATCATAGGAACATGAAGCGAGAAGTGAACCAAACTTTGGGT
GCGCCCAAGCGACTTGCCAGACAGGACCTCGATGACCGGTTAAGGTAGCTAGATGCTGAG
ATCCACCGCTGTTGCTTACTCCGGTTATCTTGATCGTGCAGTCAGACGAGGCAGTTGCTA
CTCGCTTTCCATAGTAATCCATGACCACATCA

Last Updated on 06.12.2018 16:11