Sequences from confirmation amplicon


Confirmation sequences for line 387E01 and hit At2g37420 are shown.
Line ID 387E01
Locus At2g37420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15701845
Primers used for PCR MM35 - 8474, show primer details
   
Sequence Name 16-K116018-0022-387-E01C-MM35
GenBank Accession LN493574 [GenBank]
Sequencing primer gene specific primer, MM35: 5'-CTCTTAAGTTCCAATGGGAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116018-0022-387-E01C-MM35
TTTATGTTAAGAGATAATGTGAGGCAGACGTACACTTTCACTGAGATTTAGTTCATTCTC
CAACTGTTCTATCCTCTCAATTCTCGCCTGAAACAAAGTGATCATTAAGCCCAGCATAAA
ATGAATATACACTTAAGTGTCTTCTTTTGAATCTACCTTCTTTTCAACTTCTTCTTGTGT
ATATCTTTCATGTGCAATGTAAACACCATTCTTATCCCTTGCTGCTCTTACATCTGCATT
GATTGTTTAACAATTTTAACTAATCCAATAGCTCATTTGATAATATACATAAGAAAATCT
GGAGAATATAGAACAGAATGTTGTGTTCATACCTTCTTTCATTCTCTCAAGCTCCAAGTA
AAGATCTTTAAGCAACACAGCCTTGGATAACTTCTGGTTTGCCTGCAGAGACACGTCTAG
TTAGAAGGATAGAAATTAAGGGTACATTGATAAATAAAGATGAGATATGGTAGCATAAAC
AAAACCTCAGGTTTGTTCTTGATGTTCTTGGCACGATAGGCATAATCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 03-K116017-0022-387-E01C-8409
GenBank Accession LN487630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116017-0022-387-E01C-8409
GCCTATCGTGCCAGAACTCAAGAACAAACCTGAGGTTTTGTTTATGCTACCATATCTCAT
CTTTATTTATCAATGTACCCTTAATTTCTATCCTTCTAACTAGACGTGTCTCTGCAGGCA
AACCAGAAGTTATCCAAGGCTGTGTTGCTTAAAGATCTTTACTTGGAGCTTGAGAGAATG
AAAGAAGGTATGAACACAACATTCTGTTCTATATTCTCCAGATTTTCTTATGTATATTAT
CAAATGAGCTATTGGATTAGTTAAAATTGTTAAACAATCAATGCAGATGTAAGAGCAGCA
AGGGATAAGAATGGTGTTTACATTGCACATGAAAGATATACACAAGAAGAAGTTGAAAAG
AAGGTAGATTCAAAAGAAGACACTTAAGTGTATATTCATTTTATGCTGGGCTTAATGATC
ACTTTGTTTCAGGCGAGAATTGAGAGGATAGAACAGTTGGAGAATGAACTAAATCTCAGT
GAAAGTGTACGTCTGCCTCACATTATCTCTTAACATTAAAGTGTATCTGTACGTTAGAAA
CTAAATTAATTTTCTCC

Last Updated on 06.12.2018 16:11