Sequences from confirmation amplicon


Confirmation sequences for line 387G01 and hit At4g38160 are shown.
Line ID 387G01
Locus At4g38160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17902278
Primers used for PCR DL04 - 8474, show primer details
   
Sequence Name 35-K027488-022-387-G01C-8409
GenBank Accession LN501695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K027488-022-387-G01C-8409
CCATTTACATTGAATATATCCTGTCCACTGTAGATAAGCTTCTCACTTTCAGTTCAATTG
GATAGCCGGAAAGGCTCTCCTCCAAGGTAAATTCCTCTCTACTTCCTTGTTAAAGCTCGA
ATCATCTCTAAGTTAAGCAAAATTGGTGTGTTGTAGTGGCGTTTATATGGAGGTGACAAA
TACGAGCAGCATCATGTGGTTCTTCAGGGACAAAGGTTTCGATGATCCGAGTATCGACAA
GATGTTGAGAAAATGCAAACAGTTGGAAAAGGCTCAAAGCGATGTGGCATCAGAAAACTG
GGACTACCTGAGTAACATTGTTGGTATCCAAGAGAGAAAACTCCCTTACATCGTCTCTCG
ATGCCCCAAAATCCTGACTTTACGCCTCGATGAGAGACTCATCCCGATGGTCGAGTGCCT
CTCCAGTCTTGGAAGGAATCCTCGGGAAGTTGCTTCCGCCATTACCAAATTTCCTCCAAT
ACTCTCT
   
Sequence Name 36-K027488-022-387-G01C-DL04
GenBank Accession LN502087 [GenBank]
Sequencing primer gene specific primer, DL04: 5'-TCCTCCACGCTATGAGAGAGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K027488-022-387-G01C-dl4
GCGGAGCACTTCCGAGGATTCCTTCCAGACTGGAGAGGCACTCGACCATCGGGATGAGTC
TCTCATCGAGGCGTAAAGTCAGGATTTTGGGGCATCGAGAGACGATGTAAGGGAGTTTTC
TCTCTTGGATACCAACAATGTTACTCAGGTAGTCCCAGTTTTCTGATGCCACATCGCTTT
GAGCCTTTTCCAACTGTTTGCATTTTCTCAACATCTTGTCGATACTCGGATCATCGAAAC
CTTTGTCCCTGAAGAACCACATGATGCTGCTCGTATTTGTCACCTCCATATAAACGCCAC
TACAACACACCAATTTTGCTTAACTTAGAGATGATTCGAGCTTTAACAAGGAAGTAGAGA
GGAATTTACCTTGGAGGAGAGCCTTTCCGGCTATCCAATTGAACTGAAAGTGAGAAGCTT
ATCTACAGTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11