Sequences from confirmation amplicon


Confirmation sequences for line 388C12 and hit At3g05140 are shown.
Line ID 388C12
Locus At3g05140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1437212
Primers used for PCR KP10 - 8474, show primer details
   
Sequence Name 31-K025446-022-388-C12C-8409
GenBank Accession LN500005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K025446-022-388-C12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGAAGATAAACTCGATACCTGG
ACTGAAATTGTCAGTTGCAATTTCGATGTCAGATATTGAGAAGTTTTGGAGAGATGATTG
GAAATCGTAGATATCGTGAAGGACCATGCCATTCATGTTTGGATCTGCACTTTTGTTTTG
TTTGATGATCGGTGGGGCGGCGCCACTTAATGGAGGAAAGGAAGAGAGGCGTTTCATGGA
TCTGCGTTTCCATAGCTTGAAGAATTTTCCCCAATTAGAAGATGCTCTGTGATTCTTGCA
CTGTACGTCTGTGTCTGCATCTGCATTGTCGATAGCTTCTCTGTTTGGAGGAGGATCGTC
AGATGAAGATGAAGCGGAAGAGATTGAGCAACGAGTTAAGCAGGCTTCGAGGGCTCCTCT
TGGTGATTTGGGGTCTTGCTTCTCTTTATCTACCTCTAGTAATCTTAGATCTGGGAGGAG
AAGGGAGAGAAAACGTTGGAAGAAGCTAGGTTTTTTGGTGTACAAGCAATGAATAAGAAT
ACAGAGGACAA
   
Sequence Name 32-K025446-022-388-C12C-KP10
GenBank Accession LN500429 [GenBank]
Sequencing primer gene specific primer, KP10: 5'-GCTCTGTTTTCTCTTGTCCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K025446-022-388-C12C-KP10
ATTCTTATTCATTGCTTGTACACCAAAAAACCTAGCTTCTTCCAACGTTTTCTCTCCCTT
CTCCTCCCAGATCTAAGATTACTAGAGGTAGATAAAGAGAAGCAAGACCCCAAATCACCA
AGAGGAGCCCTCGAAGCCTGCTTAACTCGTTGCTCAATCTCTTCCGCTTCATCTTCATCT
GACGATCCTCCTCCAAACAGAGAAGCTATCGACAATGCAGATGCAGACACAGACGTACAG
TGCAAGAATCACAGAGCATCTTCTAATTGGGGAAAATTCTTCAAGCTATGGAAACGCAGA
TCCATGAAACGCCTCTCTTCCTTTCCTCCATTAAGTGGCGCCGCCCCACCGATCATCAAA
CAAAACAAAAGTGCAGATCCAAACATGAATGGCATGGTCCTTCACGATATCTACGATTTC
CAATCATCTCTCCAAAACTTCTCAATATCTGACATCGAAATTGCAACTGACAATTTCAGT
CCAGGTATCGAGTTTATCTTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC

Last Updated on 06.12.2018 16:11