Sequences from confirmation amplicon


Confirmation sequences for line 390A04 and hit At2g44650 are shown.
Line ID 390A04
Locus At2g44650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18420592
Primers used for PCR 37jd - 8474, show primer details
   
Sequence Name 75-K113077-0022-390-A04C-37jd
GenBank Accession LN513271 [GenBank]
Sequencing primer gene specific primer, 37jd: 5'-ATCAAGAGCAAGTTTAGGATTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113077-0022-390-A04C-37jd
TAGAGATAGTGTCTCAATTCTTGCATCCGTATTTTCTAGGTGATAAGTTGCCATTTTTGC
CTTTAATGTTGATCTCAACATATTGTACCGTTGGTATTATGTACTCTTGGATTTGGCAGC
TCCGGAAGATCCTGACATGACATATAGATAGATTCATGTAATAGTTAACTTGATTTTAGT
ACTACGTGAGTTTTTCCCTCCGTAGTATATGACTTTTCAGTATACCCCACGATACTGCAT
TACTGCTTCGGTGACTCTCAATAAAGTGCAATTGAGATTTCTCGGTTCAATAAAACATAC
ATCGGTTAGGCTTAATAGAATAAAGATATTGGGTCTTAATAGTTAAATGGGCTGTAATAT
TGGCCCAATGTAAAACATAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 48-K113075-0022-390-A04C-8409
GenBank Accession LN506549 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K113075-0022-390-A04C-8409
TTATGTTTACATTGGGCCAATATTACGCCCATTTAACTACTATACACAATATCTTTATTC
TATTAAGCCTAACCGATGTATGTTTTATTGAACCGAGAAATCTCAATTGCACTTTATTGA
GAGTCACCGAAGCAGTAATGCAGTATCGTGGGGTATACTGAAAAGTCATATACTACGGAG
GGAAAAACTCACGTAGTACTAAAATCAAGTTAACTATTACATGAATCTATCTATATGTCA
TGTCAGGATCTTCCGGAGCTGCCAAATCCAAGAGTACATAATACCAACGGTACAATATGT
TGAGATCAACATTAAAGGCAAAAATGGCAACTTATCACCTAGAAAATACGGATGCAAGAA
TTGAGACACTATCTCTACTATAATCAAACCCATTAGATAGCTCTTCTCAAAACATCCAAT
CCTAAACTGCCTCTTGA

Last Updated on 06.12.2018 16:11