Sequences from confirmation amplicon


Confirmation sequences for line 390H09 and hit At4g37910 are shown.
Line ID 390H09
Locus At4g37910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17827239
Primers used for PCR 41qn - 8474, show primer details
   
Sequence Name 90-K115691-0022-390-H09C-41qn
GenBank Accession LN515061 [GenBank]
Sequencing primer gene specific primer, 41qn: 5'-GAAAAATAATCATATACCCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K115691-0022-390-H09C-41qn
GCAGAAGAAGAACACGGGACTACCTCAAAAACACCACTTGAGATTTCCAAGATAGAAACA
TCAAAGGTTCCACCTCCAAGATCAAACACTGCAATGACACCTTCCTTGTTGTTCATACCA
TATGATAGTGCAGCAGCTGTAGGTTCGTTGATGATCCTCTGGACATCAAGACCAGCGATT
TTCCCAGCATCCTTAGTGGCTTGTCTCTGTGCATCATTGAAATAAGCTGGAACGGTAACA
ACAGCTTTGTTGATAGATTTCCCGAGGTAAGCTTCAGCTGTTTCCTTCATTTTAGTGAGG
ACATTAGCTCCAATCTGACTGGGAGAAAACTTTTGTCCATTGGCTTCGACCCAAGCATCA
CCGTTAGGTGCCTTAACAATCTTGTAAGGAACCATTTTCATCTCCTTCTGAGTCTGTGGA
TCATCAAAACGTCTCCCAATCAAACGTTTAGAAAAAGAAATGTAAATGGCTTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 42-K115690-0022-390-H09C-8409
GenBank Accession LN504582 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115690-0022-390-H09C-8409
TCATTGAACCATTTACATTTCTTTTTCTAAACGTTTGATTGGGAGACGTTTTGATGATCC
ACAGACTCAGAAGGAGATGAAAATGGTTCCTTACAAGATTGTTAAGGCACCTAACGGTGA
TGCTTGGGTCGAAGCCAATGGACAAAAGTTTTCTCCCAGTCAGATTGGAGCTAATGTCCT
CACTAAAATGAAGGAAACAGCTGAAGCTTACCTCGGGAAATCTATCAACAAAGCTGTTGT
TACCGTTCCAGCTTATTTCAATGATGCACAGAGACAAGCCACTAAGGATGCTGGGAAAAT
CGCTGGTCTTGATGTCCAGAGGATCATCAACGAACCTACAGCTGCTGCACTATCATATGG
TATGAACAACAAGGAAGGTGTCATTGCAGTGTTTGATCTTGGAGGTGGAACCTTTGATGT
TTCTATCTTGGAAATCTCAAGTGGTGTTTTTGAGGTAGTCCCTTGTTTCTTCTTCTGCTA
TGTTATTTTTTTTTGAATTGCCTTTTCTCTTTTGTGGGT

Last Updated on 06.12.2018 16:11