Sequences from confirmation amplicon


Confirmation sequences for line 391E10 and hit At1g52470 are shown.
Line ID 391E10
Locus At1g52470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19555034
Primers used for PCR KP61 - 8474, show primer details
   
Sequence Name 48-K034066-022-391-E10C-KP61
GenBank Accession LN506428 [GenBank]
Sequencing primer gene specific primer, KP61: 5'-GTCGTAAAAGGAGTAGGAGGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K034066-022-391-E10C-KP61
GCTCTCCATTTTGCAACAAATTGTGCTCTTGGACATTATCCTATAAATCCCCGAGTTGTT
GTTGGAATTAATGGGTGGCTTTCTATTACGGGGTATATAATTTATCTTCTATTACTCATT
CACTTATCTCTATTAAGTTTTAAGAAATATTTAAATTTATTAATTACTCAAACCTCGCCA
GGAGCATAACAAGCAGCATAGAATATACAGTTGGGGCTGTAGCTCGTGCTGCATCACAGA
AAATCTTTTTTACACGTGGAGCAGGTAATCTTTATTTTCCTCTTTAGATAATGGTCACAA
GGTAATGGACATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 32-K034066-022-391-E10C-8409
GenBank Accession LN500485 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K034066-022-391-E10C-8409
AAGCCATTTACAATTGAATATGATGTCCATTACCTTGTGACCATTATCTAAAGAGGAAAA
TAAAGATTACCTGCTCCACGTGTAAAAAAGATTTTCTGTGATGCAGCACGAGCTACAGCC
CCAACTGTATATTCTATGCTGCTTGTTATGCTCCTGGCGAGGTTTGAGTAATTAATAAAT
TTAAATATTTCTTAAAACTTAATAGAGATAAGTGAATGAGTAATAGAAGATAAATTATAT
ACCCCGTAATAGAAAGCCACCCATTAATTCCAACAACAACTCGGGGATTTATAGGATAAT
GTCCAAGAGCACAATTTGTTGCAAATTGGAGAGCTACTACTGCACCCATGCCAAAGCCTC
CTACTCTTTTTACA

Last Updated on 06.12.2018 16:11