Sequences from confirmation amplicon


Confirmation sequences for line 392A01 and hit At3g50620 are shown.
Line ID 392A01
Locus At3g50620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18785775
Primers used for PCR AY50 - 8474, show primer details
   
Sequence Name 21-K025392-022-392-A01C-8409
GenBank Accession LN495509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K025392-022-392-A01C-8409
GATTTCCCGGACATGCTTCGTAAACCAATCAAGATTATAAACTCTATCAAGAGTCTGAAT
GATTGAAGAAATGTTTTTCCTTCGATCCAAAACAGAGAAAATCTCTCCATTAGAACTCAC
ATTGTTATGACTATTCAACAATGTTTCGAACCAACCGCTTCCTGATCTCTGCATCGACAA
TATCGCAAAGTACCTCACCGGATTATGTCCACATTCAGCTCTAGTTTAAGACAACCCAAA
ACAAAGATTTAAACAAAGGAACAAACCAAAGAGAGTCAAAAACATAGACAAGAACAGAAC
TGGTATATTAGTTACCTATTAAAAGTCTGAGGCTTTGGATAATGAATCCGAGTAACAAAG
CGTAGCGAGTGAGAATCAATAGGACTAGTTTGGACAAGTTGGGAGGTTTGGAAGCTGACA
TTACTTAACTGTTTTAAGCAAACAGCGCAAATGTAGAGACCACAAACCATAGCAAACACT
AAAACAATCATCCTTAAGAATAATGGTGAT
   
Sequence Name 22-K025392-022-392-A01C-AY50
GenBank Accession LN495956 [GenBank]
Sequencing primer gene specific primer, AY50: 5'-GCAACCAAAGAAATCACCATTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K025392-022-392-A01C-AY50
GATGATTGTTTTAGTGTTTGCTATGGTTTGTGGTCTCTACATTTGCGCTGTTTGCTTAAA
ACAGTTAAGTAATGTCAGCTTCCAAACCTCCCAACTTGTCCAAACTAGTCCTATTGATTC
TCACTCGCTACGCTTTGTTACTCGGATTCATTATCCAAAGCCTCAGACTTTTAATAGGTA
ACTAATATACCAGTTCTGTTCTTGTCTATGTTTTTGACTCTCTTTGGTTTGTTCCTTTGT
TTAAATCTTTGTTTTGGGTTGTCTTAAACTAGAGCTGAATGTGGACATAATCCGGTGAGG
TACTTTGCGATATTGTCGATGCAGAGATCAGGAAGCGGTTGGTTCGAAACATTGTTGAAT
AGTCATAACAATGTGAGTTCTAATGGAGAGATTTTCTCTGTTTTGGATCGAAGGAAAAAC
ATTTCTTCAATCATTCAGACTCTTGATAGAGTTTATAATCTTGATTGGTTTACGAAGCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTT

Last Updated on 06.12.2018 16:11