Sequences from confirmation amplicon


Confirmation sequences for line 392E02 and hit At1g69825 are shown.
Line ID 392E02
Locus At1g69825 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26286533
Primers used for PCR 34nt - 8474, show primer details
   
Sequence Name 61-K114626-0022-392-E02C-34nt
GenBank Accession LN510585 [GenBank]
Sequencing primer gene specific primer, 34nt: 5'-AAACTTAAGAAAAGTAGAAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K114626-0022-392-E02C-34nt
TGTCATTTAGATATTATTTCTTTTTCCTTTTTTCTTTTTATCAAAAGGTAAATTTATGGG
TGTTAAAATGAGTCAAGATATATATGGGTCAACAAAAACTCAATATATGTTTGTCTTTAA
GAAAATATAGATGTTATGGTTCAGATCGACTTAAATGGTTCATGCACACTTCAAACATTC
AAAGATGACAGAACAATGTACTAAAGTAATTTTACTTTCCTTTATATGTATGCTAATATT
TTGCCAAAAACGTCATTTTAAGATTTATTTCTAATTGTCGCAAACATGTCTTTAATTGTT
AGAAAAATATCTAATATAAAATTGGAAATGAATTAAAATTCCATAGTTGAATAAACTCTT
CTAGACCATATATATATATATATTTGGGGTCTATCAAAGACTGTAAATTGACATATTTGT
AAGAAAAAATAAAACTCAGATAAGAAAATGGCATCTAACAAAGTCTCATTCATCTTTATT
CTTTTTCTATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCC
   
Sequence Name 61-K114625-0022-392-E02C-8409
GenBank Accession LN510584 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K114625-0022-392-E02C-8409
GCCATTTACTAGAAAAGAATAAAGATGAATGAGACTTTGTTAGATGCCATTTTCTTATCT
GAGTTTTATTTTTTCTTACAAATATGTCAATTTACAGTCTTTGATAGACCCCAAATATAT
ATATATATATGGTCTAGAAGAGTTTATTCAACTATGGAATTTTAATTCATTTCCAATTTT
ATATTAGATATTTTTCTAACAATTAAAGACATGTTTGCGACAATTAGAAATAAATCTTAA
AATGACGTTTTTGGCAAAATATTAGCATACATATAAAGGAAAGTAAAATTACTTTAGTAC
ATTGTTCTGTCATCTTTGAATGTTTGAAGTGTGCATGAACCATTTAAGTCGATCTGAACC
ATAACATCTATATTTTCTTAAAGACAAACATATATTGAGTTTTTGTTGACCCATATATAT
CTTGACTCATTTTAACACCCATAAATTTACCTTTTGATAAAAAGAAAAAAGGAAAAAGAA
ATAATATCTAAATGACATTGTTGGTATTTTGATAAATTCGTAATGAAATATGTTTCTAC

Last Updated on 06.12.2018 16:11