Sequences from confirmation amplicon


Confirmation sequences for line 392F12 and hit At1g11930 are shown.
Line ID 392F12
Locus At1g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4028805
Primers used for PCR 46ns - 8474, show primer details
   
Sequence Name 38-K114649-0022-392-F12C-46ns
GenBank Accession LN503076 [GenBank]
Sequencing primer gene specific primer, 46ns: 5'-GAAGCTGCAAATTTCAATGTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114649-0022-392-F12C-46ns
ATTTTTAAGGGATTCGTAGAAATCATAGCAAGCAGAGAGAACCTGAGGTGCCTTCTCAAT
GATCTCTTGCACATAATTCTCTCCAAAAGACCTCTGACCAGCGTCGTATACTTGGCGAAT
CAGAGAAACTGGTTTCGTCTTACTCACCGCTACGACCCGTATCTGATCCGACCCACGACC
CGCTTTCTCCGCCGCCTGGTTTACCCGCTGAAACACCGATCTCAGAGCCGCCACACCATC
AATCGCCGCCGCCGACATAGTAGAATCCGCCACGTGTTCTTTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K114648-0022-392-F12C-8409
GenBank Accession LN490289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114648-0022-392-F12C-8409
AAGAACCGTGGCGGATTTACTATGTCGGCGGCGGCGATTGATGGTGTGGCGGCTCTGAGA
TCGGTGTTTCAGCGGGTAAACCAGGCGGCGGAGAAAGCGGGTCGTGGGTCGGATCAGATA
CGGGTCGTAGCGGTGAGTAAGACGAAACCAGTTTCTCTGATTCGCCAAGTATACGACGCT
GGTCAGAGGTCTTTTGGAGAGAATTATGTGCAAGAGATCATTGAGAAGGCACCTCAGGTT
CTCTCTGCTTGCTATGATTTCTACGAATCCTTTAAAAATTGAATCTTTTGGATTAGAAAA
GTTCTGAATTTATATTGATACATTGAATTGGGCAGCTT

Last Updated on 06.12.2018 16:11