Sequences from confirmation amplicon


Confirmation sequences for line 392G11 and hit At4g19865 are shown.
Line ID 392G11
Locus At4g19865 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10781549
Primers used for PCR 28qv - 8474, show primer details
   
Sequence Name 73-K115779-0022-392-G11C-28qv
GenBank Accession LN512958 [GenBank]
Sequencing primer gene specific primer, 28qv: 5'-TGGGGACAGTATACAAAACATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115779-0022-392-G11C-28qv
CCAACTCACCTCATCACACTTCTCATCGCGATCACCGCAACCAAATCTTCTTCCTATTAT
TGGGGTTATGCTTCATATATCCTTCCCATAAAAGTAAGAGTTTCCCACCACATGCAACTA
GTTTAGTCGTATTACCACTTGAGCCACCATTCCTCATGTACTTATCATCTAGTGACTCTA
ACCCTTCCAATCTTTTCCAATAATCCTTTTGTATGTCATTGTCATACCACAACAAGCGCA
TGTTCCAGAAGCCATATGAAACATTCTCAATCATACATTCTGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 25-K115778-0022-392-G11C-8409
GenBank Accession LN497596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115778-0022-392-G11C-8409
GCATTTACATTGAATATATCCGAATGTATGATTGAGAATGTTTCATATGGCTTCTGGAAC
ATGCGCTTGTTGTGGTATGACAATGACATACAAAAGGATTATTGGAAAAGATTGGAAGGG
TTAGAGTCACTAGATGATAAGTACATGAGGAATGGTGGCTCAAGTGGTAATACGACTAAA
CTAGTTGCATGTGGTGGGAAACTCTTACTTTTATGGGAAGGATATATGAAGCATAACCCC
AATAATAGGAAGAAGATTTGGTGTGCGGTGATCGCGATAGAGAAGTGTGATGGAGGTGGA
GTTTGGGGAATTGTTGAGAGTGTTGATGTTTTG

Last Updated on 06.12.2018 16:11