Sequences from confirmation amplicon


Confirmation sequences for line 393D11 and hit At3g55700 are shown.
Line ID 393D11
Locus At3g55700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20671272
Primers used for PCR 51rq - 8474, show primer details
   
Sequence Name 33-K116964-0022-393-D11C-51rq
GenBank Accession LN501152 [GenBank]
Sequencing primer gene specific primer, 51rq: 5'-TAGGGAGGTAACCCTTATCCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116964-0022-393-D11C-51rq
CGCACCACCTGTCCTCACACCACCCTACGAACTCCAATCTCTTCCGCTACAACCTCCGTG
TTCTTCCCCCATATAGCGTCGGAGACCAAACAACACACCGTCTCTCCTCCACCTACTTCC
GCCGCCACAGACTTACGAAACGGTTCGGCGTAGCGTTGTTTCAGCCGACGAACGAGAACG
ATTAGGTCCATACTCGAAGTTTCTGACTGAGACAGAGGATCTTCTTCTCCTTCTTTGTTG
TGAGAGATGGTTCGAAAAGTGAAGTGTGGGTGGCGAGAAGGATCGGGGAAGTTGTAGGAA
GTGTGGAGGATCGTCACGGAGAAGCCACGGTGGTGGAATATTCCAGCGAGCTCGATCATG
GGGTTGAAGTGCCCTGGAAATGGAAGAGGGAACAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 15-K116963-0022-393-D11C-8409
GenBank Accession LN493146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116963-0022-393-D11C-8409
TCGAGCTCGCTGGAATATTCCCCACCGNTGGCTTCTCCGTGACGATCCTCCACACTTCCT
ACAACTTCCCCGATCCTTCTCGCCACCCACACTTCACTTTTCGAACCATCTCTCACAACA
AAGAAGGAGAAGAAGATCCTCTGTCTCAGTCAGAAACTTCGAGTATGGACCTAATCGTTC
TCGTTCGTCGGCTGAAACAACGCTACGCCGAACCGTTTCGTAAGTCTGTGGCGGCGGAAG
TAGGTGGAGGAGAGACGGTGTGTTGTTTGGTCTCCGACGCTATATGGGGGAAGAACACGG
AGGTTGTAGCGGAAGAGATTGGAGTTCGTAGGGTGGTGTTGAGGACAGGTGGTGCGTCGT
CGTTTTGTGCTTTTGCCGCTTTCCCTCTCCTTAGGGATAAGGGTACCCTCCCTAAGGAGA
GGG

Last Updated on 06.12.2018 16:11