Sequences from confirmation amplicon


Confirmation sequences for line 394B09 and hit At4g31750 are shown.
Line ID 394B09
Locus At4g31750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15365118
Primers used for PCR AB57 - 8474, show primer details
   
Sequence Name 77-K105358-0022-394-B09C-AB57
GenBank Accession LN513552 [GenBank]
Sequencing primer gene specific primer, AB57: 5'-ATGAAATTTGCAATAAGTCGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K105358-0022-394-B09C-AB57
TGAATTTTTTTTTGTTTGTCAAGCTATTGCTGTATCCCGAGATCACAAGCCTGATCCATA
TGACGAGCGCCAAAGAATTGAGGATGCAGGAGGATTTGTCATGTGGGCTGGTAAGAAAAC
ATTTAACTGAAATTTCTCTTCTTATTTGTGCTTATATAATTTGCTTAATTGGAATTATAC
TAAAAGGAACATGGAGAGTTGGAGGAGTTCTTGCTGTTTCTCGTGCATTTGGCGATAGGT
TGTTGAAGCAGTATGTTGTTGCTGATCCGGAGATACAGGTATTGACTTTTTGCCAAAATC
TCCTCCTATATATTAAAAATGCCACTTTGTTATTAACAATTGAACATAACCTGCATTGGA
TTTCAATAGTTAGCTACTTGGTAAAAGAGTGACTGTTTTGTGTTACTTGATACAATCATT
CATGTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 61-K105359-0022-394-B09C-8409
GenBank Accession LN510518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K105359-0022-394-B09C-8409
TTACATTGAATAATCCGGACATGAATGATTGTATCAAGTAACACAAAACAGTCACTCTTT
TACCAAGTAGCTAACTATTGAAATCCAATGCAGGTTATGTTCAATTGTTAATAACAAAGT
GGCATTTTTAATATATAGGAGGAGATTTTGGCAAAAAGTCAATACCTGTATCTCCGGATC
AGCAACAACATACTGCTTCAACAACCTATCGCCAAATGCACGAGAAACAGCAAGAACTCC
TCCAACTCTCCATGTTCCTTTTAGTATAATTCCAATTAAGCAAATTATATAAGCACAAAT
AAGAAGAGAAATTTCAGTTAAATGTTTTCTTACCAGCCCACATGACAAATCCTCCTGCAT
CCTCAATTCTTTGGCGCTCGTCACTTTGATCAGGCTTGTGATCTCGGGATACAGCAATAG
CTTTGACAAACAAAAAAAAATATCAAATTAGTACCATACAAAACAGAGCGACTTATTGCA
AATTTC
Primers used for PCR 35ah - 3144, show primer details
   
Sequence Name 07-K106400-0022-394-B09C-3144
GenBank Accession LN489283 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106400-0022-394-B09C-3144
CCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCTAGA
ATGGCACTTTACAAAACTTCCTTCGTTCACTCATTAGCATAAATGGAAAATTTCAGGAGG
AAAAAGTTGATAGCTCTCTCGAGTTTCTCATTCTTGCAAGTGATGGTCTCTGGGACGTTG
TATCTAACGAGGTAGGAAGTGTCATTTGATTTCTAAACTTCCCTGTTAAACTTCCTCTTT
TTAACATTCCCAATCCTCGGACTCAAAAACTGATCTCTGTTCAAATCTTTATTTCAGGAA
GCCGTAGGCATGATCAAGGCGATAGAAGATCCCGAGGAAGGTGCAAAGAGACTGATGATG
GAAGCTTACCAAAGAGGAAGTGCAGACAACATAACTTGTGTCGTCGTACGTTTCTTTTCA
GACCAAGCAGGAGGAATAGGTTCCAGCAGCACCAATATCCCAATAGATCATGGTAATCGT
AC
   
Sequence Name 23-K106401-0022-394-B09C-35ah
GenBank Accession LN496567 [GenBank]
Sequencing primer gene specific primer, 35ah: 5'-GGTACGATACCATGATCTATTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106401-0022-394-B09C-35ah
GTCTGAAAGAACGTACGACGACACAGTTATGTTGTCTGCACTTCCTCTTTGGTAAGCTTC
CATCATCAGTCTCTTTGCACCTTCCTCGGGATCTTCTATCGCCTTGATCATGCCTACGGC
TTCCTGAAATAAAGATTTGAACAGAGATCAGTTTTTGAGTCCGAGGATTGGGAATGTTAA
AAAGAGGAAGTTTAACAGGGAAGTTTAGAAATCAAATGACACTTCCTACCTCGTTAGATA
CAACGTCCCAGAGACCATCACTTGCAAGAATGAGAAACTCGAGAGAGCTATCAACTTTTT
CCTCCTGAAATTTTCCATTTATGCTAATGAGTGAACGAAGGAAGTTTTGTAAAGTGCCAT
TCTAGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA

Last Updated on 06.12.2018 16:11