Sequences from confirmation amplicon


Confirmation sequences for line 395D12 and hit At1g29090 are shown.
Line ID 395D12
Locus At1g29090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10163726
Primers used for PCR NU01 - 8474, show primer details
   
Sequence Name 39-K103847-0022-395-D12C-NU01
GenBank Accession LN503329 [GenBank]
Sequencing primer gene specific primer, NU01: 5'-CTAATGTAACCATTCTCTCCCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103847-0022-395-D12C-NU01
TTGATCCCCTCTGGGCTCGTTCCGTATCCAACGAACGTCACTGCATGGTTCACGTTGGTC
CCACAGTACGGCTCATCGTACACTCCCCCTGAGTAATGCATGAAACCGGGCCCATCCGCG
TCAATGGACACTGAGACAGGCTGCTTGGATACAGCCTCGAGCAATGCACGCTCGTTGTTG
CTTGGAACGGTCTGGAACCCTCTGATCCATGCGGAGGGTTTCCCATTGTACCGGCATGTC
CCCTCTGCCGCTTGGTAAGGGTATGATGCCTCTGAGGCAATGCCTCGGTTTTTGATTATA
TAGCTGAAAGCGTCTGACATTATTCCACCGTTGCAACCGTTGTCACGCTCTCTGTCGCAA
TCTAGAAGTTGTTGTTCAGACAATGATACTAGGTTGTTTCCAACAATCTTTGTCAAACCT
TCCACCGCTGCGACGCTNGAGAATGCCCAACAACATCCTGCCCATAAATGCATCGATATA
TTGATTAGTTATATGCATAAATGCCCGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAGAAATGTAGATATCCGCA
   
Sequence Name 55-K103848-0022-395-D12C-8409
GenBank Accession LN508790 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103848-0022-395-D12C-8409
CGGACTGAGCCATTTACATTGAATATATCCGGGCATTTATGCATATAACTAATCAATATA
TCGATGCATTTATGGGCAGGATGTTGTTGGGCATTCTCAAGCGTCGCAGCGGTGGAAGGT
TTGACAAAGATTGTTGGAAACAACCTAGTATCATTGTCTGAACAACAACTTCTAGATTGC
GACAGAGAGCGTGACAACGGTTGCAACGGTGGAATAATGTCAGACGCTTTCAGCTATATA
ATCAAAAACCGAGGCATTGCCTCAGAGGCATCATACCCTTACCAAGCGGCAGAGGGGACA
TGCCGGTACAATGGGAAACCCTCCGCATGGATCAGAGGGTTCCAGACCGTTCCAAGCAAC
AACGAGCGTGCATTGCTCGAGGCTGTATCCAAGCAGCCTGTCTCAGTGTCCATTGACGCG
GATGGGCCCGGTTTCATGCATTACTCAGGGGGAGTGTACGATGAGCCGTACTGTGGGACC
AACGTGAACCATGCAGTGACGTTCGTTGGATACGGAACGAGCCCAGAGGGGATCAAGTAC
TGGCTGGCTAAAAACTCGTGGGGTGAGACTTGGGGAGAGAATGTTTACATTA

Last Updated on 06.12.2018 16:11