Sequences from confirmation amplicon


Confirmation sequences for line 396D11 and hit At2g34250 are shown.
Line ID 396D11
Locus At2g34250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14463453
Primers used for PCR 05gj - 8474, show primer details
   
Sequence Name 51-K112309-0022-396-D11C-05gj
GenBank Accession LN507548 [GenBank]
Sequencing primer gene specific primer, 05gj: 5'-TTTGAATTGATTGAGCTGTTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112309-0022-396-D11C-05gj
TTATTTTGTAGGAATGGTGCTCAGAAGCTTCTAGGTATTCTGATTGCCATTGGTGAGGCT
GTTGCATATGTTCTTTCTGGAATGTATGGTCCCGTTGGACAGCTTGGTGTTGGAAATGCC
ATTCTGATCATCCTTCAGCTTTTCTTTGCTGGAATCATTGTTATCTGCCTTGATGAACTC
CTTCAGAAGGGATATGGTCTCGGATCAGGAATCTCCCTTTTCATTGCCACCAACATTTGG
TAGGTCAACTTCTTCCTCACTTTAAAAAATCCAAAATCCTTTTCGGTATATTGAACTCAT
AAGAGTTTATATTGATGTTCTGCAGTGAATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K112308-0022-396-D11C-8409
GenBank Accession LN501883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112308-0022-396-D11C-8409
TTGAAATATTCACTGCAGAACATCAATATAAACTCTTATGAGTTCAATATACCGAAAAGG
ATTTTGGATTTTTTAAAGTGAGGAAGAAGTTGACCTACCAAATGTTGGTGGCAATGAAAA
GGGAGATTCCTGATCCGAGACCATATCCCTTCTGAAGGAGTTCATCAAGGCAGATAACAA
TGATTCCAGCAAAGAAAAGCTGAAGGATGATCAGAATGGCATTTCCAACACCAAGCTGTC
CAACGGGACCATACATTCCAGAAAGAACATATGCAACAGCCTCACCAATGGCAATCAGAA
TACCTAGAAGCTTCTGAGCACCATTCCTACAAAATAAGTACAAACACAGAATAAGAACTC
ACACGACCACAACCTTAGGGTTTTCCAAAAACAGCTCATCNAATTCAA

Last Updated on 06.12.2018 16:11