Sequences from confirmation amplicon


Confirmation sequences for line 396E04 and hit At5g44960 are shown.
Line ID 396E04
Locus At5g44960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18154191
Primers used for PCR KP53 - 8474, show primer details
   
Sequence Name 54-K034067-022-396-E04C-KP53
GenBank Accession LN508394 [GenBank]
Sequencing primer gene specific primer, KP53: 5'-GCAATAATCTGCTGTCAAATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K034067-022-396-E04C-KP53
TTTCTTCATTCCTCATCGCACGATTTTGATGTTGATGTTGTTGTTCAGGATCTAGGGATG
GAGGAGTGCGATTATATCAACGAATTGCCTGATTCTTTACTAACTCAGATACTCTTAGAC
CTTCGGACCAAAGATTCTGTTAAGACAAGCGTGTCGTCGAAGAGATGGAGAAATCTTTGG
TTAAACGTTCCTGGACTTGACTTATTCAGCCTTCAATTCACAAATCCTCATCATGAAGAA
GGCTTGATAAAGTTTATGGACAGATTTATGGAGTCTAACTGCAGGTCACGCCTGCAAAAG
TTCATGATAAGGTATTTCGAATGCAATGGTTATCGAGATCGATTCATGGAGTTGATCGGT
ACAGTGGTTGATTGTGGAATTCAACATTTAAATGGCTAAATGCTGATCGGTACAGGATAT
ATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACGTCCCAAATG
   
Sequence Name 38-K034067-022-396-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K034067-022-396-E04C-8409
AAGCCATTTACAATTGAATATATCCTGATATATCCTGTACCGATCAGCATTTAGCCATTT
AAATGTTGAATTCCACAATCAACCACTGTACCGATCAACTCCATGAATCGATCTCGATAA
CCATTGCATTCGAAATACCTTATCATGAACTTTTGCAGGCGTGACCTGCAGTTAGACTCC
ATAAATCTGTCCATAAACTTTATCAAGCCTTCTTCATGATGAGGATTTGTGAATTGAAGG
CTGAATAAGTCAAGTCCAGGAACGTTTAACCAAAGATTTCTCCATCTCTTCGACGACACG
CTTGTCTTAACAGAATCTTTGGTCCGAAGGTCTAAGAGTATCTGAGTTAGTAAAGAATCA
GGCAATTCGTTGATATAATCGCACTCCTCCATCCCTAGATCCTGAACAACAACATCAACA
TCAAAATCGTGCGATGAGGAATGAAGAAATAACCGTCTCCGTCGATTTGATTTGACAGCA
GAATTATTGCAAGACTTGGAGAAATCCTACTAGGCTTTGATTTGACAGCAGATTATTGC
Primers used for PCR KP54 - 8474, show primer details
   
Sequence Name 77-K034070-022-396-E04C-KP54
GenBank Accession LN513494 [GenBank]
Sequencing primer gene specific primer, KP54: 5'-TAGCTCTCTTTCTTGGATCCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K034070-022-396-E04C-KP54
AACTAAGAATTTGGCTCTAATTTCGATACTAAACAAGGAACTCATGTTCTTTACCATAAT
CCTATCAAATCGACTCTCGTAAAATGTCATATACTTGAGTCGTGGAGCATCAATCTCAAC
TCAAAACTCTGTACCACTCCCATTGGTCGCAAAATATAAACGAAGGCTTTTCAGAGTCTG
AGAGCTCACACGCAGAAACAGTATAACGTCCTGAGTCAAAATATCAAAAGGCTTAATCAG
CTCAAGATCTTCAAGAACAGGACATCCTGAGATGAGCTTCTCCACAACCAAAGGGCCATC
CTCACCGTAACAAATTTTCATCAGTTTCAAGATCTTGAGACAAGGTAGAGAAACAACAAA
GTCTGGATTCTTCAACTCTACGTTATAAAGCTTCAAAGAGACCAATGTCTTGCTCTTGTA
AATGTTTTGACGTATGAAATCAACTCTATTACAAGTATGCATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCC
CCAAATG
   
Sequence Name 61-K034070-022-396-E04C-8409
GenBank Accession LN510446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K034070-022-396-E04C-8409
GAAGCCATTTACAATTGAATATATGCATACTTGTAATAGAGTTGATTTCATACGTCAAAA
CATTTACAAGAGCAAGACATTGGTCTCTTTGAAGCTTTATAACGTAGAGTTGAAGAATCC
AGACTTTGTTGTTTCTCTACCTTGTCTCAAGATCTTGAAACTGATGAAAATTTGTTACGG
TGAGGATGGCCCTTTGGTTGTGGAGAAGCTCATCTCAGGATGTCCTGTTCTTGAAGATCT
TGAGCTGATTAAGCCTTTTGATATTTTGACTCAGGACGTTATACTGTTTCTGCGTGTGAG
CTCTCAGACTCTGAAAAGCCTTCGTTTATATTTTGCGACCAATGGGAGTGGTACAGAGTT
TTGAGTTGAGATTGATGCTCCACGACTCAAGTATATGACATTTTACGAGAGTCGATTTGA
TAGGATTATGGTAAAGAACATGAGTTCCTTGTTTAGTATCGAAATTAGAGCCAAATCTAG
TTTTGAGTATGGCGGTCTTTTGAAAGCAGAGGATCCAAGAAGAAGAGCTAAAAGGCCATC
TACATTTTTGAATT

Last Updated on 06.12.2018 16:11