Sequences from confirmation amplicon


Confirmation sequences for line 399E01 and hit At1g69890 are shown.
Line ID 399E01
Locus At1g69890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26323664
Primers used for PCR 48ns - 8474, show primer details
   
Sequence Name 36-K114669-0022-399-E01C-48ns
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 48ns: 5'-TTTTATCAAAGTGGGAGAGTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114669-0022-399-E01C-48ns
CTTTATATTTAAAAAAAAAAAAAACTTTTTATGTTAAAACATTTTTTTGGGGCAATTTCC
CATAAAAAGGGGGGGGACAAACTTTAATGGCTTTCTTTTACAAAAAAGCCTTTGGTCCAA
TAAATGGAAAGATTAATTCAATCCCCCAAAAATGTTCTATCGAAATTTTCTAGGATAAAT
CCCCCCCCCTTTTCTAGGGGGGGCACTTGGGCAGGGACCAACCTCTCATTTTGGACCCAA
AAACTTCACCTACAATTCTCTTTATTTAAAACTTTCAAAAAAAGGGGAACCCCCCCGCCT
GGCCTTTAATTATTGGGGGGTCCAAAAAGGACTGGCTTTGTCCCCCTATAGGGGGGAATT
TGCAAACAAAAAGAAAACCCCTGGCCTTTTAAAGGAGGGGGTGTACAAACCAAAAAATCA
CCCAAACAATTCTTTTTTGGAAACCCCGGCTTAAATGGTTCCACGTTTTTTCCTTTGAAA
TTCACCCCAATTCCAAGGGCGAATTCTCCCCCCAGGTGTCCTTTTCCTCCCGCAATGGGG
TATTCATTTGAAAAGGGCTCCAGGCCCGGAAATTCTCCGGGGATAACCATGGATTATAAG
GGTCAATAGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTCCATTTCAAAA
   
Sequence Name 13-K114668-0022-399-E01C-8409
GenBank Accession LN492149 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114668-0022-399-E01C-8409
ACTACCACATCGCGGACGAAGAAGGACACGTGGAGGAAGAATCAGCCGTTGGATATGCGT
TGACTTTCAAAGGAAATAGCGTGGAACAGTTGACGCAGGCGTTGCAAGAAGAGACTAGTA
TGGATGATGTTGTGGTCTGTACACGCCATCCTTCAAACGGCAAGCTGTTTCCTCTTCGTT
TGCAACTTCCACCTAATAGTGGAACAATGCATGTCATTCTAGTACCCTCAAGTACTTAAC
GGCAAGCTGTGTGGTTCCCCTTTATTTCGAATGTATTATATAAGTAGACTAGTAGTTGAA
GTATTAGGCTCAAGATTGAGAGTTTGGTCCTTGCCTAGTTGCCTCCATTAGATACGAGTG
TGGAATTAATCATAGAAAGTTCAGATAGACTATTATTGTGGATTTGATTTAATCTTTCAT
TTTATTGTACATAAGCTTATTTTGTAAACGAAGCATTTAAGTTTCGTCCTCCCATTTTTT
ATGTGAATTCGCCATAAAAAATTGTTTGTACTATAAAAGTTTTTTTTTTTTTTTTAAATT
TAAAGGTGAACCCCATTTTTTGCCAAGTTTTCCCCCA

Last Updated on 06.12.2018 16:11