Sequences from confirmation amplicon


Confirmation sequences for line 399G03 and hit At1g62740 are shown.
Line ID 399G03
Locus At1g62740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23231807
Primers used for PCR BB56 - 8474, show primer details
   
Sequence Name 36-K103847-0022-399-G03C-BB56
GenBank Accession LN502200 [GenBank]
Sequencing primer gene specific primer, BB56: 5'-TCATAGTACTCTTGCTGCTCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103847-0022-399-G03C-BB56
TTCAGTCTCTTCATGTTTCAGGGTTACGATGCTCCGTAAGAGCTTTCTGGTAAGTCTGAA
TCACAGGTTCATAGTCTTTCGAGACTTTAGCCATCTTCCCTAAAGCAGTTCCTTTCCTAG
TCAAAGCTTTAGCCACCATCTTGTAATCAGACCTAAGCTCTCTACCCCTTTCAACAGCCT
TATCACAATCCTTGATACACTCATCATACTAAAACAAAACACATAGACATTAAGACTCAT
AACCTAACCAAAGCAAATCACTTTAGACTAGCACACAAAAAAAAAAGAACACTTTGAAAA
CTATACCTTTCCCATCTCTGGATAAACAGCACCACGATAAGGGATATAAAAAATATCTTC
ATCATCAATCTCCATAGCTGTAGAGTAAGGCTGGATTGCAGGAAATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTCCG
   
Sequence Name 52-K103848-0022-399-G03C-8409
GenBank Accession LN507795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103848-0022-399-G03C-8409
TTCCGGACATGAGCCATTTACATTGAATATATCCTGCAATCCAGCATTACTCTACAGCTA
TGGAGATTGATGATGAAGATATCTCTTATATCACTAATCGTGCTGCTGTTCATCTAGAGA
TGGGAAAGGTATAGTTTTCAAAGTGTTCTTTTTTTTTTGTGTGCTAGTCTAAAGTGATTT
GCTTTGGTTAGGTTATGAGTCTAAAGGTCTATGTGTTTTGTTTTAGTATGATAAGTGTAT
CAGGGATTGTGATAAGGCTGTTGAAAGGGGTAAAAAGCTTAGGTCTGATTACAAAATGGT
GGCTAAAGCTTTGACTAGAAAAGGAACTGCTTTAGGGAAAATGGCTAAAGTCTCGAAAGA
CTATAAACCTGTGATTCAAACTTACCAAAAGGCTCTAACGGAGCACCGTACCCCTAAAAC
ATTGAAAAAACTGAATGAGGCAAAAAGAGCAAAAAAAGATTTGGAGCACCAAGAGTACTA
TAA

Last Updated on 06.12.2018 16:11