Sequences from confirmation amplicon


Confirmation sequences for line 400C02 and hit At1g62130 are shown.
Line ID 400C02
Locus At1g62130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22962501
Primers used for PCR 18dh - 8474, show primer details
   
Sequence Name 53-K109676-0022-400-C02C-18dh
GenBank Accession LN508182 [GenBank]
Sequencing primer gene specific primer, 18dh: 5'-ACGGTTCTTGATTTCTTCAAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K109676-0022-400-C02C-18dh
AGATCGTCCGATTGATCGAAAAGGGAAAAAACATTTGGAACTAGAGACCGAACCTGACTG
GTTTAGTTGAAATGTGAACCGTTATTAACTATTTAAGCTAAAACAAATTGTTGAAATGTG
ACTACGACAAGAATATAAGTCATTTGAAACTACCAGTGACCGAACCTGACAGGTTTGTAT
CAAAACTCCGACTAAAACCTGTTATTCTGTCAGAACTCACTATACCTTTATAGTTTATAC
TGTTTTATGACTATCTATCTATTCACGTGAACATATTATATACAACACATTCTGCTTCTG
AAGTAGACTCAATCCTACACGTACTGGCTGAATGATTCATTCCTTCTGGATCCACCTTCC
CCGTAGTCTTCGTTCCATTGTCGAAGCGCTGTCATGTTGACAGACTTTGATGAGATGCTC
ATACTCACCTGATTCAACAACATCGACAACAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGATTATTATA
   
Sequence Name 37-K109675-0022-400-C02C-8409
GenBank Accession LN502646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K109675-0022-400-C02C-8409
GCCATTTGTTGTCGATGTTGTTGAATCAGGTGAGTATGAGCATCTCATCAAAGTCTGTCA
ACATGACAGCGCTTCGACAATGGAACGAAGACTACGGGGAAGGTGGATCCAGAAGGAATG
AATCATTCAGCCAGTACGTGTAGGATTGAGTCTACTTCAGAAGCAGAATGTGTTGTATAT
AATATGTTCACGTGAATAGATAGATAGTCATAAAACAGTATAAACTATAAAGGTATAGTG
AGTTCTGACAGAATAACAGGTTTTAGTCGGAGTTTTGATACAAACCTGTCAGGTTCGGTC
ACTGGTAGTTTCAAATGACTTATATTCTTGTCGTAGTCACATTTCAACAATTTGTTTTAG
CTTAAATAGTTAATAACGGTTCACATTTCAACTAAACCAGTCAGGTTCGGTCTCTAGTTC
CAAATGTTTTTTCCCTTTTCGATCAATCGGACGATCTTTGCTTTGAAAGCATAGAAACAC
TTGAAGAAATCAAGAACCG

Last Updated on 06.12.2018 16:11