Sequences from confirmation amplicon


Confirmation sequences for line 401B01 and hit At1g48450 are shown.
Line ID 401B01
Locus At1g48450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17909421
Primers used for PCR AB59 - 8474, show primer details
   
Sequence Name 58-K105307-0022-401-B01C-AB59
GenBank Accession LN509703 [GenBank]
Sequencing primer gene specific primer, AB59: 5'-CAGCCTGATAAGTCTCTTCTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105307-0022-401-B01C-AB59
GCCTGCTGCTCATGCTAGTCTCCCTTCATCTGACCCACCTGGGAGAATCCTCATCGTCTT
CTCAAGCTGAAACCTTTGATCGATCCTCTTCAGGAAATACCCATACATCACCGAAGCAGC
ATATACCTGTCCAACTCCAAGTTTGCTTATCTGCGCAACTGCTGTCAGATCATCAGTGCG
GTTTTTAAGAATGATGGAAAGATGATTCTGAATCATCTCATAAACTTCAGGCGAGTGAAG
TCTCTCAAGTTCCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACAGATTATA
   
Sequence Name 42-K105308-0022-401-B01C-8409
GenBank Accession LN504460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K105308-0022-401-B01C-8409
GCCATTTACAATTGAGGAACTTGAGAGACTTCACTCGCCTGAAGTTTATGAGATGATTCA
GAATCATCTTTCCATCATTCTTAAAAACCGCACTGATGATCTGACAGCAGTTGCGCAGAT
AAGCAAACTTGGAGTTGGACAGGTATATGCTGCTTCGGTGATGTATGGGTATTTCCTGAA
GAGGATCGATCAAAGGTTTCAGCTTGAGAAGACGATGAGGATTCTCCCAGGTGGGTCAGA
TGAAGGTGAGACTAGCATTGAGCAAGCAGGCCGAGATGTAGAGAGAAACTTCTATGAAGA
AGCAGAAGAGACTTATCAGGCTGAAA

Last Updated on 06.12.2018 16:11