Sequences from confirmation amplicon


Confirmation sequences for line 401E04 and hit At4g31170 are shown.
Line ID 401E04
Locus At4g31170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15155129
Primers used for PCR 21uk - 8474, show primer details
   
Sequence Name 30-K117104-0022-401-E04C-21uk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21uk: 5'-TAATTGTTGTCGTTGTTGTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117104-0022-401-E04C-21uk
CAGCATCCTTTCCATCTCGTGTTGAGTGTAATGTATAATGTCTGACCGAGCTGCAAGTCA
CATGTAAATCGCTTTCAATCACTTCAACATGCACCTAAAGCAAATATAGACCATAGTAAA
GGAAACAAAAGAAAGGATATGAGAAATCACTATTATCAAAATGGTATTTTCTTTCATTGA
AGTAACTGCAAATACAAAGAACTTACCTTTAAGAGCAAATCAAGAAGCTAGAAGAAATGA
AAGTTTATGTTTCGTGAAGCTCTACACCGTCAACACCCTAAAACTGAAATTTGATCAGAA
GTAAGCTTGTGGGTTATGATCTTATTACTTTGTGGGTATTGATTTTGTCGATCGGATTCA
GAGTTTTTCTCTTTTCGATCGTTTTACTTATCCAGTACACCAAGGCAAGGTTTGCGTGTT
TCTTTTCATGTCATATCAAGTGTTTTTGTGTTTTGTGAAGCTCTAGGTAATCTATGAGTT
CTTGCTTTAGCTTATCTCATTATGGAATTATCGGATCTGTGTCTACTTTTTTGGGTTTTG
TGGGAAATGATGATTTTATTTTTTTCATCCACGAGTTTTCTCATATGACCTCTCATTTTT
CTTTGTTTTCTTTGAAATAAAATTATGGAATTCAATGATGAAGTTACTATTTTTACCTAG
TTGAAGCTGAGCTGAGTTATTAAAGCATCTGATTTCAGCTTAATTCACGAAAATTTTCAT
TCTTATACTAACATGTATATATATGTTACATGTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 14-K117103-0022-401-E04C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117103-0022-401-E04C-oriL
ATTTACATTGAATATATCCTGACATGTAACATATATATACATGTTAGTATAAGAATGAAA
ATTTTCGTGAATTAAGCTGAAATCAGATGCTTTAATAACTCAGCTCAGCTTCAACTAGGT
AAAAATAGTAACTTCATCATTGAATTCCATAATTTTATTTCAAAGAAAACAAAGAAAAAT
GAGAGGTCATATGAGAAAACTCGTGGATGAAAAAAATAAAATCATCATTTCCCACAAAAC
CCAAAAAAGTAGACACAGATCCGATAATTCCATAATGAGATAAGCTAAAGCAAGAACTCA
TAGATTACCTAGAGCTTCACAAAACACAAAAACACTTGATATGACATGAAAAGAAACACG
CAAACCTTGCCTTGGTGTACTGGATAAGTAAAACGATCGAAAAGAGAAAAACTCTGAATC
CGATCGACAAAATCAATACCCACAAAGTAATAAGATCATAACCCACAAGCTTACTTCTGA
TCAAATTTCAGTTTTAGGGTGTTGACGGTGTAGAGCTTCACGAAACATAAACTTTCATTT
CTTCTAGCTTCTTGATTTGCTCTTAAAGGTAAGTTCTTTGTATTTGCAGTTACTTCAATG
AAAGAAAATACCATTTTGATAATAGTGATTTCTCATATCCTTTCTTTTGTTTCCTTTACT
ATGGTCTATATTTGCTTTAGGTGCATGTTGAAGTGATTGAAAGCGATTTACATGTGACTT
GCAGCTCGGTCAGACATTATACATTACACTCAACACGAGATTGGAAAGGATGCTTGAGAA
TCCAAAGTTCGATTTGCACGCTGTTGGCAATCACA
Primers used for PCR 20uk - 8474, show primer details
   
Sequence Name 29-K117104-0022-401-E04C-20uk
GenBank Accession LN499454 [GenBank]
Sequencing primer gene specific primer, 20uk: 5'-AGATGATGAAGACGAAGATGACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117104-0022-401-E04C-20uk
CGATAGTGGGGATGATGACCTTAGCAGCGACGGCCATGAAAGTATTGCTCTGAAGCTGTC
CCAATTAATAAAAAAATTAGGGTTAAAATGTGTATTGCTCGGAATGGTAGAGCGCGTGTG
AGACACTTTCTATCGGAGAGAGTATTGCTCGGAAGCTGTCCCAATTAATAAAAAAACGAA
TTTGCCAGCAAAGGACGGACCAAAATTAAATAAAAATCCCAAAAAAAACAAATTAATAAT
AAAAAAAAAAAAGAGAGAAATTCGGATTCGGGAAAAAAAAATTAATGGGGAAGCGACAAC
GGACTCTTCTTCCTCCCCCTCCCCCACCTTTGGTTTAGGTTCTGGTTTTCGGGATATATC
CATTTGAAAAGGGCTTCAGGTCCGGGAAATCTCCTGGGATCACCAAGGAGTATGAGGGTC
AATATGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAAGGTAAATTTC
CC
   
Sequence Name 13-K117103-0022-401-E04C-8409
GenBank Accession LN492222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117103-0022-401-E04C-8409
TTACATTGATATATCCTGAAAACAAGAACCTAAACAGAGGTGGAGGAGGAGGAGGAAGAA
GAGTCAGTCGTCGCTTCCCATTTACTCTTTTTTTCCAGACTCCGATTTTCTCTCTTTTTT
TTTTTAATAATAAATTGGTTTTTTTGGGGATTTTTATTAAATTTGGGCCCGCCCTTGGCG
GGAAAATTCTTTTTTTTATAAATGGGGACAGCTTCCAAGCAATACTCTCCCCAAAAAAAA
GGGTCTCAAACGCGCTCTACCTTTCCAAGAAAAACACTTTTAACCCCTAATTTTTTTATT
AATGGGAACAGCTTCAAAGCAATACTTTCAGGGCCGCCCCTGCTAAGGTCATCACCCCCA
CTATCGTTTTGGTCCCCGGCTAGCCCCAAAATTCAATCCGGGTCATCTCC

Last Updated on 06.12.2018 16:11