Sequences from confirmation amplicon


Confirmation sequences for line 401F10 and hit At3g07800 are shown.
Line ID 401F10
Locus At3g07800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2490947
Primers used for PCR MC75 - 8474, show primer details
   
Sequence Name 33-K102175-0022-401-F10C-8409
GenBank Accession LN500939 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102175-0022-401-F10C-8409
GGATTTTTCTTCATTGATATATCCGGACATAGCAAGTCTCTGCCATGGCGACTCTCAAAG
CTTCCTTTTTGATCAAAACCCTCGACAGTGACGTCACCGGAGATTTTCTCTCCGATCTGG
AACGTCGTGGGTCAGGTGCTGTTCATGTTATCATGGGTCCTATGTTTTCTGGGAAATCGA
CCTCTCTCCTTCGCCGAATCAAGTCAGAGATCAGCGACGGAAGGTACAGTTTATTTTCTA
TCTCCGTAGATTTTAATTGTTAGATCTATCTCCGTAAATTTCTTAATTTCGTGTTTTGGT
TAAGCAGAAGTGTTGCGATGCTGAAATCGAGTAAGGATACGAGATACGCAAAAGATTCGG
TGGTGACACATGATGGAATTGGATTCCCTTGCTGGGCTCTTCCAGATCTCATGTCATTTC
CTGAGAAATTCGGACTAGATGCTTATAACAAGGTAATAAATTCGATGCTCTATTTGGTGT
TTCGGGTCTTAATTGTGAAGGATCTGTATTGAATTTGTGTTTTTCTCTTAAAATGATTGG
CAGCTTGATGTGATTGGTATTGATGAGGCTCAGTTCTTTGGAGATCTTTATGAGTTTTGC
TGCAAAGTCGCTGATGATGATGGTAAATGGTTGATCG
   
Sequence Name 33-K102179-0022-401-F10C-MC75
GenBank Accession LN500940 [GenBank]
Sequencing primer gene specific primer, MC75: 5'-ACGATCACAATTTTACCATCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102179-0022-401-F10C-MC75
GATCTCCAAGAACTGAGCCTCATCAATACCAATCACATCAAGCTGCCAATCATTTTAAGA
GAAAAACACAAATTCAATACAGATCCTTCACAATTAAGACCCGAAACACCAAATAGAGCA
TCGAATTTATTACCTTGTTATAAGCATCTAGTCCGAATTTCTCAGGAAATGACATGAGAT
CTGGAAGAGCCCAGCAAGGGAATCCAATTCCATCATGTGTCACCACCGAATCTTTTGCGT
ATCTCGTATCCTTACTCGATTTCAGCATCGCAACACTTCTGCTTAACCAAAACACGAAAT
TAAGAAATTTACGGAGATAGATCTAACAATTAAAATCTACGGAGATAGAAAATAAACTGT
ACCTTCCGTCGCTGATCTCTGACTTGATTCGGCGAAGGAGAGAGGTCGATTTCCCAGAAA
ACATAGGACCCATGATAACATGAACAGCACCTGACCCACGACGTTCCAGATCGGAGAGAA
AATCTCCGGTGACGTCACTGTCGAGGGTTTTGATCAAAAAGGAAGCTTTGAGAGTCGCCA
TGGCAGAGACTTGCTATGTCCGGATATATTCAATTGAAGAAAAAGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCC

Last Updated on 06.12.2018 16:11