Sequences from confirmation amplicon


Confirmation sequences for line 401H03 and hit At1g13200 are shown.
Line ID 401H03
Locus At1g13200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4508143
Primers used for PCR 28sa - 8474, show primer details
   
Sequence Name 56-K116229-0022-401-H03C-28sa
GenBank Accession LN509206 [GenBank]
Sequencing primer gene specific primer, 28sa: 5'-AGATGCTGGCTTTTGGTAATAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K116229-0022-401-H03C-28sa
AAAAATCTCGCACTTTGTGACTCCCACGTTTGGACTCGACGCATCAACAATGTACTTATC
ACAATTGTACAACCACACTAATTTGTAATCGGTGGCCTTCACGAATGCTAGATGAAAGAT
TGATTTCATCACTACATTCCACTCACGTTCAGTGGGGTTAAACTTGTGGATCAAAATCTG
AAACCCTGCCCGGAGGAAGTAGGCGGAGCCACCGTGTAGCCGGATTCACTACATATACGG
AATGTGATTTTGGGGAATGGATGCAGAAAAGGCCATCACAGCTTTCAGATATGTAGATCC
AGTTGAAGAACCCTTGAGGGAAATTGAGACGAGTAAAGGATAGAAGAGAAGCCGATTCCA
AGCAGAATAACCTAAAACCAATGTCAGTGTCTGTGTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 16-K116228-0022-401-H03C-8409
GenBank Accession LN493579 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116228-0022-401-H03C-8409
GGCTTTCTCCTTTCTATCCTTTACTCCGTCTTCAATTTTCCCTTCAAGGGTTTCTTTCAA
CTGGATTCTACATATCTGAAAGCTGTGATGGCCTTTTCTGCATCCATTCCCCAAAATCAC
ATTCCGTATATGTAGTGAATCCGGCTACACGGTGGGCTCCGCCTACTTCCTCCGGCAGGG
TTTCAGATTTTGATCCACAAGTTTAACCCCACTGAACGTGAGTGGAATGTAGTGATGAAA
TCAATCTTTCATCTAGCATTCGTGAAGGCCACCGATTACAAATTAGTGTGGTTGTACAAT
TGTGATAAGTACATTGTTGATGCGTCGAGTCCAAACGTGGGAGTCACAAAGTGCGAGATT
TTTGACTTTAGGAAAAATGCTTGGAGGTACTTGGCTTGCACTCCAAGTCATCAGATATTC
TATTACC

Last Updated on 06.12.2018 16:11