Sequences from confirmation amplicon


Confirmation sequences for line 402B12 and hit At3g60180 are shown.
Line ID 402B12
Locus At3g60180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22243967
Primers used for PCR 18qp - 8474, show primer details
   
Sequence Name 61-K115691-0022-402-B12C-18qp
GenBank Accession LN510592 [GenBank]
Sequencing primer gene specific primer, 18qp: 5'-TTGCTTGGGTAAGATGATAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K115691-0022-402-B12C-18qp
ATTCGTCTGTTGTTCAAGTTCGTTGACTATGTCTTTGTATGTATGTATATGATCATCAGA
CCCTATTGAGAGAGACTTGAGACGTTTTGTAATAAACTTATCATTACTATTTAAAAACGC
CATAGCGTGTACTTTCCGTCTCGCTTCACTTTAGAGAACCGCGTGCCGTTTAAAACATGC
ACGTTGCAACCGTTAGCAGACTACACGCCGTTTTTCTTCCTTAATTTCTCTATCTTCTTC
ACCTGAATCTTCTTCGCCTGGACTTGTCAAACTTGTGCGTGATTCATCGTCACATCGCCT
TGATCATGGAAACTCCTATCGATGCTCCGAATAAGGTGTTGTATTCACCTGTCTTTCACA
AAAACTACAGTTGATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 13-K115690-0022-402-B12C-8409
GenBank Accession LN492182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115690-0022-402-B12C-8409
TTGAATATATCCTGAATCAACTGTAGTTTTTGTGAAAGACAGGTGAATACAACACCTTAT
TCGGAGCATCGATAGGAGTTTCCATGATCAAGGCGATGTGACGATGAATCACGCACAAGT
TTGACAAGTCCAGGCGAAGAAGATTCAGGTGAAGAAGATAGAGAAATTAAGGAAGAAAAA
CGGCGTGTAGTCTGCTAACGGTTGCAACGTGCATGTTTTAAACGGCACGCGGTTCTCTAA
AGTGAAGCGAGACGGAAAGTACACGCTATGGCGTTTTTAAATAGTAATGATAAGTTTATT
ACAAAACGTCTCAAGTCTCTCTCAATAGGGTCTGATGATCATATACATACATACAAAGAC
ATAGTCAACGAACTTAAACAACAGACGAATTTATAGTTCTCTAAAACCATCACCAGAAAC
ACACTTTTAGGTTTTATCATCTANCCCAAGCA

Last Updated on 06.12.2018 16:11