Sequences from confirmation amplicon


Confirmation sequences for line 402C07 and hit At1g66490 are shown.
Line ID 402C07
Locus At1g66490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24809832
Primers used for PCR 73vi - 8474, show primer details
   
Sequence Name 21-K117806-0022-402-C07C-73vi
GenBank Accession LN495843 [GenBank]
Sequencing primer gene specific primer, 73vi: 5'-GTTCATGATGATAGATTTTGGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117806-0022-402-C07C-73vi
CATTACGAAAGCGACTTCGTTGAACCATCTATAAAGCGAGTAAGTATACTTGACCAACTC
GATATATTTAAAGTCTTTCATTGCGAAGGCTTGTTGTTATGCGTCTTCAGAGGTAACCGA
TGGCCTGTAGTGTGGAACCCTTATCTGGGGGGAACAGGGTGGATTCAACCCATAAGCGAT
TTCCACAAATATCAAGTATCAGACAAGTTTGCTTTCGGATACGAGAACAAGAACCGTAAC
TACAAAATCTTGAGGTTTCTTCGATGCTTTTGAGTACTCAGACTTTTTTGGATTTTATGA
TATCTACGATCTTAACTCTAGTGCATGGAAGGTTCTTGATGTCAATCCCGATTGTGATAT
AAAATGTGGCGTGTCTTTGAAAGGAAAAACTTACTTTTTTGCTAAAGAAATAGCAAAAGC
TCCAAATGTCAAAGATTTTTTAGTGTGTTTTGATTTTACTGCTGAGAGATTTGGACCGCG
CCTTCCTCTGCCGTTTCACTCTTGCGGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 21-K117784-0022-402-C07C-8409
GenBank Accession LN495842 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117784-0022-402-C07C-8409
ATTTACCGCAAGAGTGAACGGCAGAGGAAGGCGCGGTCCAAATCTCTCAGCAGTAAAATC
AAAACACACTAAAAAATCTTTGACATTTGGAGCTTTTGCTATTTCTTTAGCAAAAAAGTA
AGTTTTTCCTTTCAAAGACACGCCACATTTTATATCACAATCGGGATTGACATCAAGAAC
CTTCCATGCACTAGAGTTAAGATCGTAGATATCATAAAATCCAAAAAAGTCTGAGTACTC
AAAAGCATCGAAGAAACCTCAAGATTTTGTAGTTACGGTTCTTGTTCTCGTATCCGAAAG
CAAACTTGTCTGATACTTGATATTTGTGGAAATCGCTTATGGGTTGAATCCACCCTGTTC
CCCCCAGATAAGGGTTCCACACTACAGGCCATCGGTTACCTCTGAAGACGCATAACAACA
AGCCTTCGCAATGAAAGACTTTAAATATATCGAGTTGGTCAAGTATACTTACTCGCTTTA
TAGATGGTTCAACGAAGTCGCTTTCGTAATTGAGTCCTTGGAGATCGAACTTTATTGAAT
AAAGCCCAAAATCTATATCCATGAAC

Last Updated on 06.12.2018 16:11