Sequences from confirmation amplicon


Confirmation sequences for line 403A07 and hit At4g04860 are shown.
Line ID 403A07
Locus At4g04860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2460136
Primers used for PCR 13ww - 8474, show primer details
   
Sequence Name 33-K119110-0022-403-A07C-13ww
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 13ww: 5'-ATAAGCAATCAATCAGTCACAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K119110-0022-403-A07C-13ww
TGACAAGACAAGAGCTTCATATGAAAGTAATAAGCACACAAATGTTGTATCCAGTTTCAT
AGAGTAGTGTTAAGGGAAAATTACCCATCTTCCGGAAATACAAGAAATTAGTAACGAGAC
GCCAATATTGGTATTGCTTCACCACAAGTGTCGGATTCAAATAAAGGTTATATGGAGATA
TGATCTGCAAAACAAAAACAATCAGAATATGAAACCCTAATTTAGAGAATTTCAGAGAAC
CCCAAAAAAGATTCGCGAATAAGCAAGCAAGCACGTACATCGAGGGAACATCCGACGGTG
GTGATAACAGCCGCCGTGAGATAAGAGCGAGTGATAATCGGCATCTGTTTATACCATTCT
TCTACAGCCTGAGCCAATATGGATATAATCGGCATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGAACGC
   
Sequence Name 10-K119109-0022-403-A07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119109-0022-403-A07C-8409
CATTTCATTGAATTGCCGATTATATCCATATTGGCTCAGGCTGTAGAAGAATGGTATAAA
CAGATGCCGATTATCACTCGCTCTTATCTCACGGCGGCTGTTATCACCACCGTCGGATGT
TCCCTCGATGTACGTGCTTGCTTGCTTATTCGCGAATCTTTTTTGGGGTTCTCTGAAATT
CTCTAAATTAGGGTTTCATATTCTGATTGTTTTTGTTTTGCAGATCATATCTCCATATAA
CCTTTATTTGAATCCGACACTTGTGGTGAAGCAATACCAATATTGGCGTCTCGTTACTAA
TTTCTTGTATTTCCGGAAGATGGGTAATTTTCCCTTAACACTACTCTATGAAACTGGATA
CAACATTTGTGTGCTTATTACTTTCATATGAAGCTCTTGTCTTTGTCACATTATTAGCTC
TAGGCTGCTACTTGTTAATAGCGTTGTGACTGATTGATTGCTTA

Last Updated on 06.12.2018 16:11