Sequences from confirmation amplicon


Confirmation sequences for line 403C01 and hit At5g48485 are shown.
Line ID 403C01
Locus At5g48485 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19646350
Primers used for PCR BF54 - 8474, show primer details
   
Sequence Name 26-K029911-022-403-C01C-8409
GenBank Accession LN497789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K029911-022-403-C01C-8409
CCATTTACAATTGTAATTACAGTGTGGTCTAGCCAACGCCCCAACTTGTTAAAAGACTCT
GTATACGTGTGTTTATGTTTTTTATTACTCCTATTCTAAATATCGGATGTTATTAATAAA
TCGTATTTTCTGCCAAATGGCGTTTATGGGTTAACCCCAAAAACTGGTCAAAAGAAAAGA
AAGAGGACCAATCGAAAACTACACAAGGCCCAAACACGGAAGGGCAATTTCGAAAAATAT
GTAACCAATAAGCGATAGAGAGAAACCACAAAGCACAAATACAGAGGTTTATGGTTTCAC
TACCTCTGACACAGTATTCAACACTCTGACACCAAAACAACAAATATTCTTAATTCCGTC
TTATCTAATCACTAACCATTTCATTTTCATTCCAAATAGACGATTATACCCTTAGTACTA
AACCAACAAAAATGACAAAGAACACAGATTAAACACAGAACGATTATAGAAGATAGATAG
AGAAGCGAAATCATCAACAAAACATAATATAGCCCGAGATTAATATGCAATTTCACCAAG
ATTAGAGTTTGTCAAAAGGGGTGAAATTAGAATCCGAAAGTCATT
   
Sequence Name 26-K029911-022-403-C01C-BF54
GenBank Accession LN497790 [GenBank]
Sequencing primer gene specific primer, BF54: 5'-GATCGAGTAGGCTAATGACTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K029911-022-403-C01C-BF54
AACTCTAATCTTGGTGAAATTGCATATTAATCTCGGGCTATATTATGTTTTGTTGATGAT
TTCGCTTCTCTATCTATCTTCTATAATCGTTCTGTGTTTAATCTGTGTTCTTTGTCATTT
TTGTTGGTTTAGTACTAAGGGTATAATCGTCTATTTGGAATGAAAATGAAATGGTTAGTG
ATTAGATAAGACGGAATTAAGAATATTTGTTGTTTTGGTGTCAGAGTGTTGAATACTGTG
TCAGAGGTAGTGAAACCATAAACCTCTGTATTTGTGCTTTGTGGTTTCTCTCTATCGCTT
ATTGGTTACATATTTTTCGAAATTGCCCTTCCGTGTTTGGGCCTTGTGTAGTTTTCGATT
GGTCCTCTTTCTTTTCTTTTGACCAGTTTTTGGGGTTAACCCATAAACGCCATTTGGCAG
AAAATACGATTTATTAATAACATCCGATATTTAGAATAGGAGTAATAAAAAACATAAACA
CACGTATACAGAGTCTTTTAACAAGTTGGGGCGTTGGCTAGACCACACTGTAATTACAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
A

Last Updated on 06.12.2018 16:11